机构：[a]Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[b]Institutes of Neuroscience, Soochow University, Suzhou 215123, China[c]Beijing Key Laboratory for Parkinson's Disease, Beijing 100053, China[d]Center for Circadian Clock, Soochow University, Suzhou, China[e]School of Biology & Basic Medical Sciences, Medical College, Soochow University, Suzhou 215123, Jiangsu, China
Vesicular monoamine transporter 2 (Vmat2) is widely distributed in the central nervous system, and responsible for uptaking transmitters into the vesicles. However, whether Vmat2-deficiency is related to the anxiety is rarely investigated, especially in zebrafish. Here, we reported Vmat2 heterzygous mutant zebrafish displayed anxiety-like behavior. The mutants spent less time in the top area and took longer latency to the top in the novel tank test. Consistently, they showed dark avoidance in the light/dark box test, with longer duration in the light zone and increased number of crossing between the two zones. Monoamine concentration analysis showed that the levels of monoamine neurotransmitters including dopamine (DA), 5-hydroxy tryptamine (5-HT) and norepinephrine (NE), as well as their metabolites were decreased in VMAT mutants. Taken together, these findings suggest that Vmat2 heterzygous mutant zebrafish may serve as a new model of anxiety, which may be related with the low level of DA, 5-HT and NE. (C) 2016 Elsevier Inc. All rights reserved.
基金：
This work was supported by the National Natural Science
Foundation of China (81471299), Jiangsu Provincial Special Program
of Medical Science (BL2014042), Suzhou Clinical Key Disease
Diagnosis and Treatment Technology Foundation (LCZX201304),
the Plans for Graduate Research and Innovation in Colleges and
Universities of Jiangsu Province (CXZZ13_0833), Suzhou Clinical
Research Center of Neurological Disease (Szzx201503) and Suzhou
Medical Key Discipline Project. This was also partly supported by
the Priority Academic Program Development of Jiangsu Higher
Education Institutions (PAPD).
第一作者机构：[a]Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
通讯作者：
通讯机构：[*1]Department of Neurology, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China.
推荐引用方式(GB/T 7714)：
Yali Wang,Siyue Li,Wenwen Liu,et al.Vesicular monoamine transporter 2 (Vmat2) knockdown elicits anxiety-like behavior in zebrafish[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2016,470(4):792-7.doi:10.1016/j.bbrc.2016.01.079.
APA：
Yali Wang,Siyue Li,Wenwen Liu,Fen Wang,Li-Fang Hu...&Chun-Feng Liu.(2016).Vesicular monoamine transporter 2 (Vmat2) knockdown elicits anxiety-like behavior in zebrafish.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,470,(4)
MLA：
Yali Wang,et al."Vesicular monoamine transporter 2 (Vmat2) knockdown elicits anxiety-like behavior in zebrafish".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 470..4(2016):792-7
