机构：[a]Department of Neurology,Second Affiliated Hospital of Soochow University,1055 Sanxiang Road, Suzhou 215004[b]Institute of Neuroscience,Soochow University, Suzhou , PR China
Objectives: Oxidized low-density lipoprotein (ox-LDL) may induce autophagy, apoptosis, necrosis or proliferation of cultured endothelial cells depending on the concentration and exposure time. Our previous studies found that ox-LDL exposure for 6 h increases the autophagic level of human umbilical vein endothelial cells (HUVECs) in a concentration-dependent manner. The present study investigates the relationship between autophagy and apoptosis in HUVECs exposed to ox-LDL. Methods: Flow cytometry and Western blot were used to study the apoptotic and autophagic phenomena. The contribution of autophagic and apoptotic mechanisms to ox-LDL-induced upregulation of MAP1-LC3, beclin1 and p53 protein levels were assessed by pretreatment with the autophagic inhibitors 3-MA and Atg5 small interfering (si) RNA, as well as z-vad-fmk, an apoptosis inhibitor. Results: ox-LDL induced the apoptosis of HUVECs in a concentration-dependent way. The increased expression of the autophagic proteins, LC3-II and beclin1, can be reversed by 3-MA and z-vad-fmk pretreatment. 3-MA and Atg5 siRNA increased the ox-LDL-induced increases of the p53 protein level and the annexin V-positive staining, which was decreased by z-vad-fmk. Conclusion: These results suggest that overstimulation of ox-LDL can induce autophagy and apoptosis in HUVECs. Inhibition of apoptosis leads to an inhibition of autophagy induced by ox-LDL. However, inhibition of autophagy leads to an increase in the ox-LDL-induced apoptosis. (C) 2015 S. Karger AG, Basel
基金：
This work was supported by a grant from the PhD
Programs Foundation of the Ministry of Education of China
(20113201120016), the Jiangsu Province Natural Science Fund of
China (BK2012172), the National Natural Science Fund of China
(81200894, 81200893), and a project funded by the Priority Academic
Program Development of Jiangsu Higher Education Institutions.
第一作者机构：[a]Department of Neurology,Second Affiliated Hospital of Soochow University,1055 Sanxiang Road, Suzhou 215004
通讯作者：
通讯机构：[a]Department of Neurology,Second Affiliated Hospital of Soochow University,1055 Sanxiang Road, Suzhou 215004
推荐引用方式(GB/T 7714)：
Yanlin Zhang,Ying Xie,Shoujiang You,et al.Autophagy and Apoptosis in the Response of Human Vascular Endothelial Cells to Oxidized Low-Density Lipoprotein[J].CARDIOLOGY.2015,132(1):27-33.doi:10.1159/000381332.
APA：
Yanlin Zhang,Ying Xie,Shoujiang You,Qiao Han,Yongjun Cao...&Chunfeng Liu.(2015).Autophagy and Apoptosis in the Response of Human Vascular Endothelial Cells to Oxidized Low-Density Lipoprotein.CARDIOLOGY,132,(1)
MLA：
Yanlin Zhang,et al."Autophagy and Apoptosis in the Response of Human Vascular Endothelial Cells to Oxidized Low-Density Lipoprotein".CARDIOLOGY 132..1(2015):27-33
