机构：[1]Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affi liated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009[2]Systems Biology Division, Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang 310029[3]Department of Neurosurgery and Brain Tumor Research Laboratory, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China[4]Swedish Neuroscience Institute, Swedish Medical Center, Seattle, WA 98122[5]Department of Urology, University of Washington, Seattle, WA 98195, USA
MicroRNAs (miRNAs) are small non-coding RNAs of 20-25 nucleotides in length that are capable of modulating gene expression post-transcriptionally. The potential roles of miRNAs in the tumorigenesis of glioblastoma (GBM) have been under intensive studies in the past few years. In the present study, we found a positive correlation between the levels of miR-127-3p and the cell migration and invasion abilities in several human GBM cell lines. We showed that miR-127-3p promoted cell migration and invasion of GBM cells using in vitro cell lines and in vivo mouse models. We identified SEPT7, a known tumor-suppressor gene that has been reported to suppress GBM cell migration and invasion, as a direct target of miR-127-3p. SEPT7 was able to partially abrogate the effect of miR-127-3p on cell migration and invasion. In addition, microarray analysis revealed that miR-127-3p regulated a number of migration and invasion-related genes. Finally, we verified that miR-127-3p affected the remodeling of the actin cytoskeleton mediated by SEPT7 in GBM cells.
基金：
This study was funded by grant 81072060 from the National Natural Science Foundation of China; grants 2008DFA11320 and 2012AA022705 from the Ministry of Science and Technology, China; grant 20110101120153 from the Ministry of Education, China; grant 2012R10021 from the Zhejiang Provincial Government; grant 2011ZX09307-001-05 from National Science and Technology Major Project, China
第一作者机构：[1]Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affi liated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009[2]Systems Biology Division, Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang 310029
通讯作者：
通讯机构：[1]Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affi liated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009[*1]Division of Systems Biology, Zhejiang-California International Nanosystems Institute (ZCNI), Zhejiang University, 268 Kaixuan Road, Hangzhou, Zhejiang 310029, P.R. China
推荐引用方式(GB/T 7714)：
HUAWEI JIANG,DASONG HUA,JING ZHANG,et al.MicroRNA-127-3p promotes glioblastoma cell migration and invasion by targeting the tumor-suppressor gene SEPT7[J].ONCOLOGY REPORTS.2014,31(5):2261-9.doi:10.3892/or.2014.3055.
APA：
HUAWEI JIANG,DASONG HUA,JING ZHANG,QING LAN,QIANG HUANG...&BIAOYANG LIN.(2014).MicroRNA-127-3p promotes glioblastoma cell migration and invasion by targeting the tumor-suppressor gene SEPT7.ONCOLOGY REPORTS,31,(5)
MLA：
HUAWEI JIANG,et al."MicroRNA-127-3p promotes glioblastoma cell migration and invasion by targeting the tumor-suppressor gene SEPT7".ONCOLOGY REPORTS 31..5(2014):2261-9
