机构：[1]Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, People’s Republic of China[2]Department of Clinical Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, People’s Republic of China首都医科大学附属天坛医院
Aim: To investigate the anticancer effects of Jinlong capsule (JLC) against human glioblastoma cells and the possible underlying mechanism. Methods: Cell Counting Kit-8 and colony formation assay were adopted for the analysis of cell viability. Cell invasion and migration were evaluated by transwell and wound healing assays. Then, the expression level of mammalian target of rapamycin (mTOR), phosphorylated mTOR (p-mTOR), S6 and phosphorylated S6 (p-S6) were determined by western blotting. Results: The results showed that JLC significantly inhibited human glioblastoma cell proliferation, invasion and migration in a dose-dependent manner. The expressions of p-mTOR and p-S6 were dramatically suppressed by JLC. Furtherly, inhibition of mTOR reduced the cell migration and invasion, while the mTOR agonist (MHY1485) could partially reverse the anti-migration and anti-invasion activity of JLC. Conclusion: The above results suggested that JLC would be a potential candidate for the treatment of glioblastoma.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81572474]; Science and Technology Development Fund Project of Traditional Chinese Medicine of Beijing [JJ2015-14]
第一作者机构：[1]Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, People’s Republic of China
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, People’s Republic of China[*1]Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, 8 Jingshun Dongjie, Beijing 100015, People’s Republic of China
推荐引用方式(GB/T 7714)：
Shi Jingren,Zhang Wenli,He Lu,et al.Jinlong capsule inhibits migration and invasion in human glioblastoma cells via the modulation of mTOR/S6 signaling pathway[J].DRUG DESIGN DEVELOPMENT AND THERAPY.2019,13:1023-1032.doi:10.2147/DDDT.S195409.
APA：
Shi, Jingren,Zhang, Wenli,He, Lu,Kong, Fanhong,Pan, Meichen...&Wang, Yajie.(2019).Jinlong capsule inhibits migration and invasion in human glioblastoma cells via the modulation of mTOR/S6 signaling pathway.DRUG DESIGN DEVELOPMENT AND THERAPY,13,
MLA：
Shi, Jingren,et al."Jinlong capsule inhibits migration and invasion in human glioblastoma cells via the modulation of mTOR/S6 signaling pathway".DRUG DESIGN DEVELOPMENT AND THERAPY 13.(2019):1023-1032
