机构：[1]Department of General Surgery, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, P. R. China[2]Department of General Surgery, First Affiliated Hospital of Soochow University, Suzhou, 215006, P.R. China
B7-H3 (B7 homologue 3, CD276) is a member of the B7 immunoregulatory family and promotes tumor progression. The present study demonstrated that B7-H3 promotes gastric cancer cell migration and invasion. shRNA-mediated B7-H3 silencing in the N87 gastric cancer cell line suppressed cell migration and invasion in vitro and in vivo; downregulated metastasis-associated CXCR4; and inhibited AKT, ERK, and Jak2/Stat3 phosphorylation. B7-H3-silenced cells injected into the tail veins of 4-week-old female BALB/c nude mice produced fewer metastases than control cells, and resulted in longer survival times. Immunofluorescence analyses confirmed B7H3/ CXCR4 colocalization in N87 cells, and co-immunoprecipitation assays showed a direct interaction between the two proteins. Our analysis of 120 tissue samples from gastric cancer patients showed that increased B7-H3 expression correlated positively with both tumor infiltration depth and CXCR4 expression. These findings suggest that B7-H3 and CXCR4 may be novel targets for anti-gastric cancer therapeutics.
基金：
This work was supported in part by the National Natural Science Foundation of China (No.81672970 and 8130193), Jiangsu Province’s Clinical Medical Science and Technology Program (No. BL2013016), the Provincial Natural Science Foundation of Jiangsu Province (No. BK20161225), the Natural Science Foundation of Jiangsu Provincial University, China (No.13KJB320018), the Postdoctoral Science Foundation Grant of China (No. 2016M591913), the Focus of Clinical Disease Treatment Technology Special Funds of Suzhou City (No. LCZX201505), Jiangsu Province’s Graduate Student Research Innovation Project (No. KYLX16_0162), the Science and Technology Program of Suzhou City, China (No. SYS201539), and the Second Affiliated Hospital of Soochow University Preponderant Clinic Discipline Group Project Funding.
第一作者机构：[1]Department of General Surgery, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, P. R. China
共同第一作者：
通讯作者：
通讯机构：[1]Department of General Surgery, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, P. R. China[2]Department of General Surgery, First Affiliated Hospital of Soochow University, Suzhou, 215006, P.R. China
推荐引用方式(GB/T 7714)：
Yecheng Li,Xiaodong Yang,Yong Wu,et al.B7-H3 promotes gastric cancer cell migration and invasion[J].ONCOTARGET.2017,8(42):71725-71735.doi:10.18632/oncotarget.17847.
APA：
Yecheng Li,Xiaodong Yang,Yong Wu,Kui Zhao,Zhenyu Ye...&Chungen Xing.(2017).B7-H3 promotes gastric cancer cell migration and invasion.ONCOTARGET,8,(42)
MLA：
Yecheng Li,et al."B7-H3 promotes gastric cancer cell migration and invasion".ONCOTARGET 8..42(2017):71725-71735
医学