机构：[1]Department of Oncology, the Second Affiliated Hospital of Soochow University, Suzhou, China[2]Department of Breast and Gynecologic Oncology, the Affiliated Hospital of Guizhou Medical University, Guizhou Cancer Hospital, Guiyang, China[3]Department of Interventional Radiology, the Affiliated Hospital of Guizhou Medical University, Guiyang, China[4]The Key Laboratory of Medical Molecular Biology, GuiZhou Medical University, Guiyang, China
OBJECTIVE: BNIP1, a member of the BH3-only protein family, plays essential roles in a variety of biological processes. How ever, the mechanism and function of BNIP1 are still unknown in cervical cancer. We aim to explore the roles of BNIP1 on cervical cancer cell proliferation, apoptosis, migration, and invasion abilities by mTOR signaling pathway. PATIENTS AND METHODS: qRT-PCR and Western blot assays were performed to assess BNIP, mTOR, and p70S6K1 expressions. CCK-8, transwell and flow cytometry assays were used to measure the representative proliferation, migration, invasion, and apoptosis abilities. RESULTS: Our findings indicated that BNIP1 is down-expressed in cervical cancer tissues and cells, and was negatively associated with lymphatic metastasis. Overexpression of BNIP1 suppressed proliferation, migration and invasion, and promoted apoptosis of cervical cancer cells. Silence of BNIP1 by siRNAs accelerated proliferation, migration and invasion, and inhibited apoptosis of cervical cancer cells. In addition, we found that BNIP1 significantly inhibited mTOR, p70S6K1, and p-p70S6K1 expressions; BNIP1 affected the proliferation, apoptosis, migration, and invasion abilities of cervical cancer cells by regulating mTOR expression. CONCLUSIONS: BNIP1 can be considered a marker for cervical carcinoma therapy.
基金：
Guizhou Province Science and Technology Fund (J for Scientific in Guizhou) [[2016]7399, [2015]7377]
第一作者机构：[1]Department of Oncology, the Second Affiliated Hospital of Soochow University, Suzhou, China[2]Department of Breast and Gynecologic Oncology, the Affiliated Hospital of Guizhou Medical University, Guizhou Cancer Hospital, Guiyang, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Oncology, the Second Affiliated Hospital of Soochow University, Suzhou, China[4]The Key Laboratory of Medical Molecular Biology, GuiZhou Medical University, Guiyang, China
推荐引用方式(GB/T 7714)：
F.-H. LI,L. XIANG,L. RAN,et al.BNIP1 inhibits cell proliferation, migration and invasion, and promotes apoptosis by mTOR in cervical cancer cells[J].EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES.2019,23(4):1397-1407.doi:10.26355/eurrev_201902_17096.
APA：
F.-H. LI,L. XIANG,L. RAN,S. ZHOU,Z. HUANG...&W.-F. YU.(2019).BNIP1 inhibits cell proliferation, migration and invasion, and promotes apoptosis by mTOR in cervical cancer cells.EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,23,(4)
MLA：
F.-H. LI,et al."BNIP1 inhibits cell proliferation, migration and invasion, and promotes apoptosis by mTOR in cervical cancer cells".EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 23..4(2019):1397-1407
