Introduction: Deep venous thrombosis (DVT) is one of the common peripheral vascular diseases. The recruitment and migration of bone marrow-derived endothelial progenitor cells (EPCs) to the sites of venous thrombus are necessary in the process of thrombus organization and recanalization. Our objective was to investigate the functional role of miR-150 in rat EPCs and its potential application in deep venous thrombosis. Materials and Methods: Rat EPCs were cultured and transfected with miR-150 mimics and inhibitor. Wound healing assay, transwell migration assay and matrigel tube formation assay were performed to elucidate the effect of miR-150 of rat EPCs. Lentiviral construct expressing miR-150 was transfected into EPCs and the EPCs were injected to rat models of DVT. The rats were sacrificed on the day of 7 and 14 after the transplantation and the histological study was performed. Luciferase reporter assay and Western blot were performed to evaluate rat miR-150 regulates the expression of c-Myb. Results: MiR-150 significantly promoted the migration and tube formation ability of EPCs in vitro and enhanced EPCs' homing, organization and resolution ability in vivo. Overexpression of miR-150 significantly reduced the protein level of c-Myb and repressed the activity of a luciferase reporter containing both of the two predicted miR-150 binding sites in c-Myb 3'-UTR, indicating that c-Myb may be a miR-150 target gene. Conclusion: MiR-150 enhanced the migration, tube formation, homing, thrombus recanalization and resolution ability of rat EPCs. Restoring miR-150 in EPCs revealed potential application in DVT therapy. (C) 2014 Elsevier Ltd. All rights reserved.
基金：
This work was supported by grants from the National Natural
Science Foundation of China (30972941), Jiangsu Provincial Health
Department’s Medical Science Program (H201211), Science and
Technology Innovation Program of Suzhou Science Institution
(SYS201234). Graduate Research and Innovation Program in
Colleges and Universities of Jiangsu Province (CXLX12-0840,
CX2211-0121).
第一作者机构：[1]Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, 215000, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Vascular Surgery, the Second Affiliated Hospital of Soochow University, No.1055, Sanxiang Rd, Suzhou 215000, Jiangsu, China.
推荐引用方式(GB/T 7714)：
Wenbin Wang,Chenglong Li,Wendong Li,et al.MiR-150 enhances the motility of EPCs in vitro and promotes EPCs homing and thrombus resolving in vivo[J].THROMBOSIS RESEARCH.2014,133(4):590-8.doi:10.1016/j.thromres.2013.12.038.
APA：
Wenbin Wang,Chenglong Li,Wendong Li,Lingshang Kong,Aimin Qian...&Xiaoqiang Li.(2014).MiR-150 enhances the motility of EPCs in vitro and promotes EPCs homing and thrombus resolving in vivo.THROMBOSIS RESEARCH,133,(4)
MLA：
Wenbin Wang,et al."MiR-150 enhances the motility of EPCs in vitro and promotes EPCs homing and thrombus resolving in vivo".THROMBOSIS RESEARCH 133..4(2014):590-8
