Study on Biodistribution and Radioimmunoimaging of (131)Iodine-Labeled Monoclonal Antibody D-D3 Against Progastrin-Releasing Peptide((31-98)) in Tumor-Bearing Mouse
机构：[1]Department of Nuclear Medicine, The Second Affiliated Hospital of Soochow University, Suzhou, China.[2]Department of Radiology and Nuclear Medicine, Chinese Institute for Radiation Protection, Taiyuan, China.[3]Department of Pathology, University of California San Diego Moores Cancer Center, La Jolla, California.
This study was aimed at investigating the biodistribution and radioimmunoimaging of I-131-D-D3 in nude mice bearing different types of tumor xenografts. Radioiodination of the D-D3 antibody was performed with the chloramine-T method. The radiochemical purity was determined through thin-layer chromotography. I-131-D-D3 was injected into healthy Kunming mice via a tail vein, and the %ID/g for various organs was obtained. Similarly, the %ID/g and tumor/nontumor tissue ratio of I-131-D-D3 in nude mice bearing small cell lung cancer (SCLC) xenografts were obtained. Planar images of I-131-D-D3 in tumor-bearing nude mice were acquired at different times after injection. The I-131-D-D3 labeling rate was 86.56% +/- 3.8%. The radiochemical purity of I-131-DD3 was 99.27% +/- 0.6%. After 12 hours of incubation in 37 degrees C water bath, the radiochemical purity was 97.64% +/- 0.5% and remained at 88.38% +/- 0.4% after 48 hours. After being mixed with healthy human serum for 24 hours, the radiochemical purity was more than 64%. The metabolism of I-131-D-D3 in healthy Kunming mice was consistent with a two-compartment model with first-order absorption; T-1/2 alpha and T-1/2 beta were 0.25 and 37.89 hours, respectively. The %ID/g of I-131-D-D3 in SCLC xenografts was much higher than those of other tissues at 48 hours after injection, and the tumor/nontumor tissue ratio also gradually increased with time. After 24 hours of injection, planar imaging was obtained, which clearly showed a contrasting tumor on the right armpit of nude mice bearing SCLC with high concentrations of radioactivity. Also, nude mice bearing gastric cancer showed similar results as that of the SCLC with a lower radioactivity level. No observable accumulation was observed in nude mice bearing pancreatic cancer or lung adenocarcinoma. The labeling rate and radiochemical purity of (ID)-I-131-D3 were high and stable. I-131-D-D3 selectively accumulated at tumors that highly expressed progastrin-releasing peptide; therefore, it is a promising radioimmunoimaging reagent for SCLC.
基金：
This study was funded by the Science and Technology
Development Planning (Suzhou, China, 2009; Grant ID:
YJS0926).
语种：
外文
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PubmedID：
中科院(CAS)分区：
出版当年[2010]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区肿瘤学4 区药学4 区核医学
最新[2023]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区肿瘤学4 区药学4 区核医学
JCR分区：
出版当年[2009]版：
Q3RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGINGQ3ONCOLOGYQ3PHARMACOLOGY & PHARMACYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q2RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGINGQ3MEDICINE, RESEARCH & EXPERIMENTALQ3ONCOLOGYQ3PHARMACOLOGY & PHARMACY
第一作者机构：[1]Department of Nuclear Medicine, The Second Affiliated Hospital of Soochow University, Suzhou, China.[*1]Department of Nuclear Medicine, The Second Affiliated Hospital of Soochow University,1055 Sanxiang Road, Suzhou 215004, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Nuclear Medicine, The Second Affiliated Hospital of Soochow University,1055 Sanxiang Road, Suzhou 215004, China
推荐引用方式(GB/T 7714)：
Zengli Liu,Xiaolin Zhou,Yizhen Shi,et al.Study on Biodistribution and Radioimmunoimaging of (131)Iodine-Labeled Monoclonal Antibody D-D3 Against Progastrin-Releasing Peptide((31-98)) in Tumor-Bearing Mouse[J].CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS.2011,26(2):229-35.doi:10.1089/cbr.2010.0855.
APA：
Zengli Liu,Xiaolin Zhou,Yizhen Shi,Shouying Du,Yi Yang...&Yongmei Shen.(2011).Study on Biodistribution and Radioimmunoimaging of (131)Iodine-Labeled Monoclonal Antibody D-D3 Against Progastrin-Releasing Peptide((31-98)) in Tumor-Bearing Mouse.CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,26,(2)
MLA：
Zengli Liu,et al."Study on Biodistribution and Radioimmunoimaging of (131)Iodine-Labeled Monoclonal Antibody D-D3 Against Progastrin-Releasing Peptide((31-98)) in Tumor-Bearing Mouse".CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS 26..2(2011):229-35
