机构：[a]Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, People’s Republic of China[b]National Center for Nanoscience and Technology (NSNST), Beijing 100190, People’s Republic of China[c]University of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China[d]State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, People’s Republic of China[e]Department of Cell Biology, Xuanwu Hospital, Capital Medical University, Beijing 100053, People’s Republic of China首都医科大学宣武医院
Bointerface mediated physical cues have been found to be able to guide stem cells to differentiate into specific cell lineages. In this study, nanostructured MoS2 biointerface was fabricated by assembling few-layer MoS2 nanoflakes onto substrate using a facile hydrothermal method. Its effects on proliferation, adhesion and osteogenic differentiation of rat bone marrow mesenchymal stem cells (MSCs) were compared with a flat substrate. The live-dead staining and cell count kit-8 (CCK-8) were used to evaluate the biocompatibility. Focal adhesion formation was used to explore the interaction between MSCs and the nanostructured MoS2. Alkaline phosphatase (ALP) activity, alizarin red S staining, immunofluorescent staining and real quantitative polymerase chain reaction (RT-qPCR) were performed to assess the osteogenic differentiation of MSCs. The results showed that the nanoporous structured MoS2 interfacecan not only promote the MSCs attachment and spreading, but also accelerate the MSCs osteogenesis. Our results can provide insight into the design considerations of the biointerface to control the stem cell fate. (c) 2017 Published by Elsevier Ltd.
基金：
the National Natural ScienceFoundation of China (No. 81471784, No. 31270022),
the YouthInnovation Promotion Association of the Chinese Academy of Sci-ences (2015023),
Nature Science Foundation of Beijing (2172058),
the “Thousands Talents Program” for pioneer researcher andhis innovation team, China
第一作者机构：[a]Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, People’s Republic of China[b]National Center for Nanoscience and Technology (NSNST), Beijing 100190, People’s Republic of China[c]University of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[a]Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, People’s Republic of China
推荐引用方式(GB/T 7714)：
Xiaodi Zhang,Jinhui Nie,Xixi Yang,et al.Nanostructured molybdenum disulfide biointerface for adhesion and osteogenic differentiation of mesenchymal stem cells[J].APPLIED MATERIALS TODAY.2018,10:164-172.doi:10.1016/j.apmt.2017.12.001.
APA：
Xiaodi Zhang,Jinhui Nie,Xixi Yang,Zhirong Liu,Weibo Guo...&Linlin Li.(2018).Nanostructured molybdenum disulfide biointerface for adhesion and osteogenic differentiation of mesenchymal stem cells.APPLIED MATERIALS TODAY,10,
MLA：
Xiaodi Zhang,et al."Nanostructured molybdenum disulfide biointerface for adhesion and osteogenic differentiation of mesenchymal stem cells".APPLIED MATERIALS TODAY 10.(2018):164-172
