机构：[1]Department of Neurobiology, School of Basic Medicine, Anhui Medical University, Hefei, Anhui 230032, China[2]Department of Anatomy, School of Basic Medicine, Anhui Medical University, Hefei, Anhui 230032, China[3]Cell Therapy Center, Xuanwu Hospital, Capital Medical University, Beijing 100053, China首都医科大学宣武医院
17 beta-Estradiol (E2) is a multifunctional steroid hormone in modulating metabolism in vivo. Previous studies have reported that E2 could promote insulin secretion and protect beta cells from apoptosis. In this study, the partial pancreatectomy (PPx) model was used to study the role of E2 in islet cell proliferation. The animals were divided into four groups, including sham control, PPx model, E2, and E2 plus estrogen antagonist (E2 plus ICI) groups. In the E2 group, 5-bromo-20-deoxyuridine-and Ki67-positive cells significantly increased after PPx, and the protein expression of forkhead transcription factor M1, cyclin A2, cyclin B1, and cyclin E2 also significantly increased in the isolated islets. The messenger RNA expression of cyclin A2 and cyclin B2 increased in E2 treatment group. Additionally, the effects of E2 on the PPx mice were partially blocked by estrogen antagonist ICI182,780. The results indicated that E2 significantly promoted islet cell proliferation in PPx model mice, and it upregulated the expression of cell cycle genes. In conclusion, E2 treatment is beneficial for islet cell proliferation in adult mice after PPx. A partial pancreatectomy in mice may be an attractive model for the study of islet cell proliferation. Copyright (c) 2017 Endocrine Society This article has been published under the terms of the Creative Commons Attribution Non-Commercial, No-Derivatives License (CC BY-NC-ND).
基金：
National Natural Science Foundation of China Grant 81372693.
Anhui Natural Science Foundation Grant 1308085MH119,
the Youth Training Program supported by First Affiliated Hospital of Anhui Medical University Grant 2010KJ17.
语种：
外文
被引次数：
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PubmedID：
第一作者：
第一作者机构：[1]Department of Neurobiology, School of Basic Medicine, Anhui Medical University, Hefei, Anhui 230032, China[2]Department of Anatomy, School of Basic Medicine, Anhui Medical University, Hefei, Anhui 230032, China
通讯作者：
通讯机构：[*1]Department of Anatomy, School of Basic Medicine, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230022, China.
推荐引用方式(GB/T 7714)：
Tingting Wu ,Jinyong Xu ,Shengchun Xu ,et al.17 beta-Estradiol Promotes Islet Cell Proliferation in a Partial Pancreatectomy Mouse Model[J].JOURNAL OF THE ENDOCRINE SOCIETY.2017,1(7):965-979.doi:10.1210/js.2016-1073.
APA：
Tingting Wu,,Jinyong Xu,,Shengchun Xu,,Lianzhong Wu,,Youyu Zhu,...&Zhenhua Ren.(2017).17 beta-Estradiol Promotes Islet Cell Proliferation in a Partial Pancreatectomy Mouse Model.JOURNAL OF THE ENDOCRINE SOCIETY,1,(7)
MLA：
Tingting Wu,,et al."17 beta-Estradiol Promotes Islet Cell Proliferation in a Partial Pancreatectomy Mouse Model".JOURNAL OF THE ENDOCRINE SOCIETY 1..7(2017):965-979
