机构：[1]Neuroelectrophysiological Laboratory, Xuanwu Hospital, Capital Medical University, Beijing, China,首都医科大学宣武医院[2]Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing, China,[3]Center of Epilepsy, Center for Brain Disorders Research, Capital Medical University, Beijing, China,[4]College of Electrical and Control Engineering, North China University of Technology, Beijing, China,[5]Core Facilities Center, Capital Medical University, Beijing, China,[6]Department of Pathology, Xuanwu Hospital, Capital Medical University, Beijing, China病理科首都医科大学宣武医院
The therapy of focal epilepsy remains unsatisfactory for as many as 25% of patients. The photolysis of caged-gamma-aminobutyric acid (caged-GABA) represents a novel and alternative option for the treatment of intractable epilepsy. Our previous experimental results have demonstrated that the use of blue light produced by light-emitting diode to uncage ruthenium-bipyridine-triphenylphosphine-c-GABA (RuBi-GABA) can rapidly terminate paroxysmal seizure activity both in vitro and in vivo. However, the optimal concentration of RuBi-GABA, and the intensity of illumination to abort seizures, remains unknown. The aim of this study was to explore the optimal anti-seizure effects of RuBi-GABA by using implantable fibers to introduce blue light into the neocortex of a 4-aminopyridine-induced acute seizure model in rats. We then investigated the effects of different combinations of RuBi-GABA concentrations and light intensity upon seizure. Our results show that the anti-seizure effect of RuBi-GABA has obvious concentration and light intensity dependence. This is the first example of using an implantable device for the photolysis of RuBi-GABA in the therapy of neocortical seizure, and an optimal combination of RuBi-GABA concentration and light intensity was explored. These results provide important experimental data for future clinical translational studies.
基金：
the National Natural Science Foundations of China (81471391, 81671367),
Beijing Natural Science Foundation (KZ201610025027),
Beijing Institute for Brain Disorders Foundation (BIBDPXM2014_014226_000016),
Innovation Center for Brain Disorders Foundation (PXM2015_014226_000051).
第一作者机构：[1]Neuroelectrophysiological Laboratory, Xuanwu Hospital, Capital Medical University, Beijing, China,[2]Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing, China,[3]Center of Epilepsy, Center for Brain Disorders Research, Capital Medical University, Beijing, China,
通讯作者：
通讯机构：[1]Neuroelectrophysiological Laboratory, Xuanwu Hospital, Capital Medical University, Beijing, China,[2]Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing, China,[3]Center of Epilepsy, Center for Brain Disorders Research, Capital Medical University, Beijing, China,
推荐引用方式(GB/T 7714)：
Dan Wang,Zhixin Yu,Jiaqing Yan,et al.Photolysis of caged-gaBa rapidly Terminates seizures In Vivo: concentration and light intensity Dependence[J].FRONTIERS IN NEUROLOGY.2017,8(MAY):215.doi:10.3389/fneur.2017.00215.
APA：
Dan Wang,Zhixin Yu,Jiaqing Yan,Fenqin Xue,Guoping Ren...&Xiaofeng Yang.(2017).Photolysis of caged-gaBa rapidly Terminates seizures In Vivo: concentration and light intensity Dependence.FRONTIERS IN NEUROLOGY,8,(MAY)
MLA：
Dan Wang,et al."Photolysis of caged-gaBa rapidly Terminates seizures In Vivo: concentration and light intensity Dependence".FRONTIERS IN NEUROLOGY 8..MAY(2017):215
