机构：[1]Central Laboratory, Xuanwu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer’s Disease, Beijing Institute for Brain Disorders, Beijing, China[2]Department of Geriatrics, Xuanwu Hospital, Capital Medical University, Beijing, China老年医学科[3]Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China神经内科[4]Shenzhen Anqun Biotech CO., Ltd., Shenzhen, China[5]Department of Neurology, Beijing Chaoyanmen Hospital, Beijing, China[6]Department of Clinical Laboratory, Xuanwu Hospital, Capital Medical University, Beijing, China检验科
BackgroundAlzheimer's disease (AD) is an age-related chronic degenerative disease that damages the nervous system. A noninvasive and simple method for early detection of AD is extremely important for the diagnosis and prognosis of AD. Thus, we aimed to develop an enzyme-linked immunosorbent assay (ELISA) kit to detect urine Alzheimer-associated neuronal thread protein (AD7C-NTP), and to evaluate its clinical value for the diagnosis of AD. MethodsImmunogenic AD7C-NTP peptide fragments were synthesized by the solid-phase method and used for immunizing mice or rabbits to generate anti-AD7C-NTP antibodies. The urine AD7C-NTP ELISA kit was then established; the generated mouse anti-AD7C-NTP antibody was used as a capture antibody, the biotin-labeled rabbit anti-AD7C-NTP antibody was used as a detection antibody, and avidin labeled by horseradish peroxidase was used as a substrate. The first morning urine specimens of 121 AD patients and 118 age-matched controls were collected, and the urine AD7C-NTP levels were detected by the above ELISA kit. ResultsMouse and rabbit anti-AD7C-NTP antibody ELISA titer was found to be 1:8,000 and 1:32,000, respectively. A single band with a relative molecular mass of 41 kDa was found in human brain specimens by Western blot assay, which was identified as AD7C-NTP antibody. The urine AD7C-NTP concentration of the AD patients was higher than that of the age-matched controls, the sensitivity was 89.3% and the specificity was 84.7%. ConclusionsOur study demonstrated that our newly developed urine AD7C-NTP ELISA kit has suggested potential for diagnosing AD in a Chinese population, suggesting it may be a useful diagnostic kit for detecting early AD.
基金：
Beijing Natural Science FoundationBeijing Natural Science Foundation [7132044]; Capital Health Research and Development of Special [2011-1001-02, 2014-1-1031]; Beijing Municipal Health Bureau Research Fund [Jing 13-02]
第一作者机构：[1]Central Laboratory, Xuanwu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer’s Disease, Beijing Institute for Brain Disorders, Beijing, China[2]Department of Geriatrics, Xuanwu Hospital, Capital Medical University, Beijing, China
通讯作者：
通讯机构：[*1]Xuanwu Hospital, Capital Medical University,[*2]Xuanwu Hospital, Capital Medical University
推荐引用方式(GB/T 7714)：
Lina Ma ,Rong Wang ,Ying Han ,et al.Development of a Novel Urine Alzheimer-Associated Neuronal Thread Protein ELISA Kit and Its Potential Use in the Diagnosis of Alzheimer's Disease[J].JOURNAL OF CLINICAL LABORATORY ANALYSIS.2016,30(4):308-14.doi:10.1002/jcla.21856.
APA：
Lina Ma,,Rong Wang,,Ying Han,,Shuli Sheng,,Jianan Zhu,...&Peichang Wang.(2016).Development of a Novel Urine Alzheimer-Associated Neuronal Thread Protein ELISA Kit and Its Potential Use in the Diagnosis of Alzheimer's Disease.JOURNAL OF CLINICAL LABORATORY ANALYSIS,30,(4)
MLA：
Lina Ma,,et al."Development of a Novel Urine Alzheimer-Associated Neuronal Thread Protein ELISA Kit and Its Potential Use in the Diagnosis of Alzheimer's Disease".JOURNAL OF CLINICAL LABORATORY ANALYSIS 30..4(2016):308-14
