机构：[1]Central Laboratory, Xuan Wu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Beijing 100053, China首都医科大学宣武医院
Diabetic encephalopathy is one of the risk factors for Alzheimer's disease. Our previous findings indicated that animals with diabetic encephalopathy exhibit learning and memory impairment in addition to hippocampal neurodegeneration, both of which are ameliorated with amyloid precursor protein (APP) 17-mer (APP17) peptide treatment. Although APP17 is neuroprotective, it is susceptible to enzymatic degradation. Derived from the active sequence structure of APP17, we have previously structurally transformed and modified several APP5-mer peptides (APP328-332 [RERMS], APP 5). We have developed seven different derivatives of APP5, including several analogs. Results from the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on human neuroblastoma SH-SY5Y cells in the present study showed that P165 was the most neuroprotective APP5 derivative. Furthermore, we tested the effects of APP5 and P165 on the number of cells and the release of lactate dehydrogenase. Western immunoblot analyses were also performed. The digestion rates of P165 and APP5 were determined by the pepsin digestion test. P165 resisted pepsin digestion significantly more than APP5. Therefore, P165 may be optimal for oral administration. Overall, these findings suggest that P165 may be a potential drug for the treatment of diabetic encephalopathy. (C) 2015 Elsevier Inc. All rights reserved.
基金：
the following grants: BeijingNatural Science Foundation (7132033, 7132044),
Capital HealthDevelopment Research Fund (2011-1001-02),
Beijing HealthSystems, Health and Technical Personnel of High-Level Plan (2009-3-64).
第一作者机构：[1]Central Laboratory, Xuan Wu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Beijing 100053, China
共同第一作者：
通讯作者：
通讯机构：[*1]Central Laboratory, Xuan Wu Hospital, Capital Med-ical University, #45 Changchun Street, Xicheng District, Beijing 100053, China.
推荐引用方式(GB/T 7714)：
Jie Yao,Lina Ma,Rong Wang,et al.Neurotrophic effects of amyloid precursor protein peptide 165 in vitro[J].BRAIN RESEARCH BULLETIN.2016,120:58-62.doi:10.1016/j.brainresbull.2015.11.005.
APA：
Jie Yao,Lina Ma,Rong Wang,Shuli Sheng,Zhijuan Ji&Jingyan Zhang.(2016).Neurotrophic effects of amyloid precursor protein peptide 165 in vitro.BRAIN RESEARCH BULLETIN,120,
MLA：
Jie Yao,et al."Neurotrophic effects of amyloid precursor protein peptide 165 in vitro".BRAIN RESEARCH BULLETIN 120.(2016):58-62
