机构:[1]Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing, China首都医科大学宣武医院低氧医学研究室[2]China-America Institute of Neuroscience, Beijing Luhe Hospital, Capital Medical University, Beijing, China中美神经科学研究所首都医科大学宣武医院[3]Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine, Beijing, China[4]Division of Pharmaceutical Sciences, Duquesne University, Pittsburgh, PA, USA
Aims: Remote ischemic preconditioning (RIPC) of a limb is a clinically feasible strategy to protect against ischemia-reperfusion injury after stroke. However, the mechanism underlying RIPC remains elusive. Methods: We generated a rat model of noninvasive RIPC by four repeated cycles of brief blood flow constriction (5 min) in the hindlimbs using a tourniquet. Blood was collected 1 h after preconditioning and 3 days after brain reperfusion. The impact of RIPC on immune cell and cytokine profiles prior to and after transient middle cerebral artery occlusion (MCAO) was assessed. Results: Remote ischemic preconditioning protects against focal ischemia and preserves neurological functions 3 days after stroke. Flow cytometry analysis demonstrated that RIPC ameliorates the post-MCAO reduction of CD3(+)CD8(+) T cells and abolishes the reduction of CD3(+)/CD161a(+) NKT cells in the blood. In addition, RIPC robustly elevates the percentage of B cells in peripheral blood, thereby reversing the reduction in the B-cell population after stroke. RIPC also markedly elevates the percentage of CD43(+)/CD172a(+) noninflammatory resident monocytes, without any impact on the percentage of CD43(-)/CD172a(+) inflammatory monocytes. Finally, RIPC induces IL-6 expression and enhances the elevation of TNF-alpha after stroke. Conclusion: Our results reveal dramatic immune changes during RIPC-afforded neuroprotection against cerebral ischemia.
基金:
the National Science Fund for Distinguished Young Scholars (No. 81325007),
American Heart Association Scientist Development Grant 13SDG 14570025
the Beijing Postdoctoral Foundation (No. 2014ZZ)
第一作者机构:[1]Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing, China[2]China-America Institute of Neuroscience, Beijing Luhe Hospital, Capital Medical University, Beijing, China[3]Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine, Beijing, China
通讯作者:
通讯机构:[*1]Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.[*2]China-America Institute of Neuroscience, Beijing Luhe Hospital, Capital Medical University, Beijing 101100, China.
推荐引用方式(GB/T 7714):
Zong-Jian Liu ,Chen Chen ,Xiao-Rong Li ,et al.Remote Ischemic Preconditioning-Mediated Neuroprotection against Stroke is Associated with Significant Alterations in Peripheral Immune Responses[J].CNS NEUROSCIENCE & THERAPEUTICS.2016,22(1):43-52.doi:10.1111/cns.12448.
APA:
Zong-Jian Liu,,Chen Chen,,Xiao-Rong Li,,Yuan-Yuan Ran,,Tao Xu,...&Xiao-Ming Hu.(2016).Remote Ischemic Preconditioning-Mediated Neuroprotection against Stroke is Associated with Significant Alterations in Peripheral Immune Responses.CNS NEUROSCIENCE & THERAPEUTICS,22,(1)
MLA:
Zong-Jian Liu,,et al."Remote Ischemic Preconditioning-Mediated Neuroprotection against Stroke is Associated with Significant Alterations in Peripheral Immune Responses".CNS NEUROSCIENCE & THERAPEUTICS 22..1(2016):43-52