机构：[a]Center for Neurodegenerative Disease, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, #6 Tian Tan Xi Li Street, 100050 Beijing, China[b]Department of Neurobiology, Xuanwu Hospital of Capital Medical University, 100053 Beijing, China首都医科大学宣武医院[c]Parkinson’s Disease Center, Beijing institute for brain disorders, #10 You’an Men Wai Xi Tou Tiao, 100069 Beijing, China[d]China National Clinical Research Center for Neurological Diseases, #6 Tian Tan Xi Li Street, 100050 Beijing, China
Parkinson's disease (PD) is a neurodegenerative disorder characterized by intracellular alpha-synuclein (alpha-syn) deposition. Alterations in alpha-syn levels in cerebrospinal fluid (CSF) and plasma of PD patients have been thought to be potential PD biomarkers; however, contamination arising from hemolysis often influences the accuracy of detecting alpha-syn levels in the CSF and plasma. In this study, alpha-syn oligomer levels in red blood cells (RBCs) obtained from 100 PD patients, 22 MSA patients, and 102 control subjects were measured by enzyme-linked immunosorbent assay. We showed that the ratio of alpha-syn oligomer/total RBC protein was higher in PD patients than in controls (29.0 +/- 19.8 ng/mg vs. 15.4 +/- 7.4 ng/mg, P < 0.001). The area under the receiver operating characteristic curve (AUC) indicated a sensitivity of 79.0%, specificity of 64.7% and a positive predictive value of 68.7%, with an AUC of 0.76 for increased alpha-syn oligomer/total RBC protein ratio. However, there was no correlation between RBC alpha-syn oligomer levels and age at onset, disease duration, age, UPDRS motor scale score or progression of motor degeneration in PD patients. The ratio of RBC alpha-syn oligomer/total protein was also higher in MSA patients than in controls (22.9 +/- 13.9 ng/mg vs. 15.4 7.4 ng/mg, P < 0.001). However, no significant difference was found for alpha-syn oligomer/total protein ratio between PD and MSA (29.0 19.8 ng/mg vs. 22.9 13.9 ng/mg, P>0.05). The present results suggest that the RBC alpha-syn oligomer/total protein ratio can be a potential diagnostic biomarker for PD. (C) 2015 Published by Elsevier Ireland Ltd.
基金：
Beijing Municipal Science and Technology Commission (grant no. Z111107058811012),
High Level Technical Personnel Training Project of Beijing HealthSystem (grant no. 2011-3-022),
Beijing Nature Science Foundation and Municipal Commission of Education (grant no.kz20120025028),
National Key Technology Research and Development Program of the Ministry of Science and Technology of China(grant no. 2013BAI09B03),
Beijing Institute for Brain Disorders (grant no. BIBD-PXM2013 014226 07 000084).
第一作者机构：[a]Center for Neurodegenerative Disease, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, #6 Tian Tan Xi Li Street, 100050 Beijing, China
通讯作者：
通讯机构：[a]Center for Neurodegenerative Disease, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, #6 Tian Tan Xi Li Street, 100050 Beijing, China
推荐引用方式(GB/T 7714)：
Xuemei Wang,Shun Yu,Fangfei Li,et al.Detection of alpha-synuclein oligomers in red blood cells as a potential biomarker of Parkinson's disease[J].NEUROSCIENCE LETTERS.2015,599:115-119.doi:10.1016/j.neulet.2015.05.030.
APA：
Xuemei Wang,Shun Yu,Fangfei Li&Tao Feng.(2015).Detection of alpha-synuclein oligomers in red blood cells as a potential biomarker of Parkinson's disease.NEUROSCIENCE LETTERS,599,
MLA：
Xuemei Wang,et al."Detection of alpha-synuclein oligomers in red blood cells as a potential biomarker of Parkinson's disease".NEUROSCIENCE LETTERS 599.(2015):115-119
