机构：[a]Department of Geriatrics, Xuan Wu Hospital, Capital Medical University, Beijing 100053, China老年医学科首都医科大学宣武医院[b]Central Laboratory, Xuan Wu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer’s Disease, Beijing Institute for Brain Disorders, Beijing 100053, China首都医科大学宣武医院[c]Department of Neurology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
Aims: To determine the effects and underlying molecular mechanisms of caloric restriction (CR) in C57BL/6 mice. Methods: Thirty-six 6-week-old male C57BL/6 mice were assigned to a normal control group (NC, n=12), a high energy group (HE, n=12), and a CR group (n= 12), and received a normal diet, a high-calorie diet, or a calorie-restricted diet, respectively, for 44 weeks. Body weight and serum glucose concentration were regularly recorded, and animals were sacrificed and hippocampus tissues were collected for immunohistochemistry (n = 6 per group), western blotting (n = 3 per group) and real-time polymerase chain reaction (n = 3 per group) analysis at the end of the 44-week experimental period. Immunohistochemistry, western blotting and real-time polymerase chain reaction were used to detect changes in hippocampal proteins may be involved in the SIRT1/mTOR pathways. Results: Body weight and serum glucose over the 44 weeks in animals from the CR group were lower than those of HE group. The number of SIRT1-immunoreactive cells in the CR group was significantly higher than in the NC and HE groups, and SIRT1 mRNA expression in the CR group was significantly higher than that in the HE group, but there was no difference in SIRT1 protein expression among the three groups. mTOR and S6K1 protein activation and mTOR and S6K1 mRNA were significantly lower in the CR group than in the NC group. Conclusions: Our findings suggest that a CR diet could lead to activation of SIRT1 and suppression of mTOR and S6K1 activation in C57BL/6 mice. We have shown that the SIRT1/mTOR signaling pathways may be involved in the neuroprotective effect of CR. (C). 2015 Elsevier Inc. All rights reserved.
基金：
the following grants: Beijing Natural Science Foundation (No. 7132044),
The Capital Health Research and Development of Special (No. 2011-1001-02, No. 2014-1-1031),
Beijing Municipal Health Bureau Research Fund (Jing 13-02)
Beijing Excellent Talent Fund (20140000204400001).
第一作者机构：[a]Department of Geriatrics, Xuan Wu Hospital, Capital Medical University, Beijing 100053, China[b]Central Laboratory, Xuan Wu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer’s Disease, Beijing Institute for Brain Disorders, Beijing 100053, China
通讯作者：
通讯机构：[*1]Central Laboratory, Xuan Wu Hospital, Capital Medical University,
推荐引用方式(GB/T 7714)：
Lina Ma,Wen Dong,Rong Wang,et al.Effect of caloric restriction on the SIRT1/mTOR signaling pathways in senile mice[J].BRAIN RESEARCH BULLETIN.2015,116:doi:10.1016/j.brainresbull.2015.06.004.
APA：
Lina Ma,Wen Dong,Rong Wang,Yun Li,Baolei Xu...&Yulan Wang.(2015).Effect of caloric restriction on the SIRT1/mTOR signaling pathways in senile mice.BRAIN RESEARCH BULLETIN,116,
MLA：
Lina Ma,et al."Effect of caloric restriction on the SIRT1/mTOR signaling pathways in senile mice".BRAIN RESEARCH BULLETIN 116.(2015)
