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Caloric Restriction Reprograms the Single-Cell Transcriptional Landscape of Rattus Norvegicus Aging.

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收录情况： ◇ SCIE ◇ 自然指数

作者：
Shuai Ma[1,2] * ; Shuhui Sun[2] * ; Lingling Geng[3,4] * ; Moshi Song[1,5,6] * ; Wei Wang[1,6] ; Yanxia Ye[3,5] ; Qianzhao Ji[1,6] ; Zhiran Zou[1,6] ; Si Wang[1,4,5] ; Xiaojuan He[4] ; Wei Li[4] ; Concepcion Rodriguez Esteban[7] ; Xiao Long[8] ; Guoji Guo[9] ; Piu Chan[4] ; Qi Zhou[3,5,6] ; Juan Carlos Izpisua Belmonte[7] ; Weiqi Zhang[4,5,6,10,11] ; Jing Qu[3,5,6] ; Guang-Hui Liu[1,2,4,5,6] ;
机构： [1]State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [2]National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China [3]State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [4]Advanced Innovation Center for Human Brain Protection, and National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing 100053, China [5]Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China [6]University of Chinese Academy of Sciences, Beijing 100049, China [7]Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA [8]Division of Plastic Surgery, Peking Union Medical College Hospital, Beijing 100032, China [9]Center for Stem Cell and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China [10]Disease Genomics and Individualized Medicine Laboratory, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China [11]China National Center for Bioinformation, Beijing 100101, China
出处：
DOI： 10.1016/j.cell.2020.02.008
ISSN：

关键词： aging caloric restriction immune cell inflammation single-cell RNA atlas single-cell RNA sequencing single-nucleus RNA sequencing

摘要：
Aging causes a functional decline in tissues throughout the body that may be delayed by caloric restriction (CR). However, the cellular profiles and signatures of aging, as well as those ameliorated by CR, remain unclear. Here, we built comprehensive single-cell and single-nucleus transcriptomic atlases across various rat tissues undergoing aging and CR. CR attenuated aging-related changes in cell type composition, gene expression, and core transcriptional regulatory networks. Immune cells were increased during aging, and CR favorably reversed the aging-disturbed immune ecosystem. Computational prediction revealed that the abnormal cell-cell communication patterns observed during aging, including the excessive proinflammatory ligand-receptor interplay, were reversed by CR. Our work provides multi-tissue single-cell transcriptional landscapes associated with aging and CR in a mammal, enhances our understanding of the robustness of CR as a geroprotective intervention, and uncovers how metabolic intervention can act upon the immune system to modify the process of aging. Copyright © 2020 Elsevier Inc. All rights reserved.

基金：
We thank Zhiheng He for his help in rat food intake. We thank Kai Du and Chunyu Chen from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences for their help in animal breeding. We thank Jinghui Lei and Jingyi Jia for assisting with animal experiments. We thank Yifang He, Yaobin Jing, Zunpeng Liu, Zehua Wang, Jinghui Lei, Xuling Han, Yiyuan Zhang, Guoxing Liu, Xing Zhang, Yaxin Wang, Jingyi Jia, Liyun Zhao, and Zeming Wu for participating in the single-cell isolation from specific rat tissues. We thank Yandong Zheng and Jiaming Li for bioinformatic analysis. We are also grateful to Lei Bai, Ruijun Bai, Jing Lu, Qun Chu, Ying Yang, May Schwarz, and Shikun Ma for administrative assistance, to Junying Jia from Institute of Biophysics, Chinese Academy of Sciences for the fluorescence-activated cell sorting (FACS), and to Junfeng Hao from Institute of Biophysics, Chinese Academy of Sciences for image scanning and analysis of Hematoxylin and eosin (H&E) staining. We would like to thank Hezhen Shan for her assistance with frozen tissue sections. We thank Prof. Zhengwei Xie for his advice on manuscript writing. This work was supported by the National Key Research and Development Program of China ( 2018YFC2000100 ), the Strategic Priority Research Program of the Chinese Academy of Sciences ( XDA16010100 ), the National Key Research and Development Program of China ( 2018YFA0107203 , 2017YFA0102802 , 2017YFA0103304 ), the National Natural Science Foundation of China ( 81921006 , 81625009 , 91749202, 81861168034 , 31671429 , 91949209 , 81671377 , 91749123 , 81771515 , 31601158 , 81701388 , 81601233 , 31601109 , 81822018 , 81870228 , 81801399 , 31801010 , 81801370 , 81922027 , 31900523 , 81901432 , 81901433 , 31900524 ), Program of Beijing Municipal Science and Technology Commission ( Z191100001519005 ), Beijing Natural Science Foundation ( Z190019 ), Beijing Municipal Commission of Health and Family Planning ( PXM2018_026283_000002 ), Advanced Innovation Center for Human Brain Protection ( 3500-1192012 ), K.C. Wong Education Foundation ( GJTD-2019-06 , GJTD-2019-08 ), the Key Research Program of the Chinese Academy of Sciences ( KFZD-SW-221 ), the State Key Laboratory of Stem Cell and Reproductive Biology , and the State Key Laboratory of Membrane Biology . J.C.I.B. was supported by the Moxie Foundation and the Glenn Foundation .
语种：
外文
高被引：
被引次数：
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PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类 | 1 区 生物
小类 | 1 区 生化与分子生物学 1 区 细胞生物学
最新[2023]版：
大类 | 1 区 生物学
小类 | 1 区 生化与分子生物学 1 区 细胞生物学
JCR分区：
出版当年[2018]版：
Q1 CELL BIOLOGY Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
最新[2023]版：
Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Q1 CELL BIOLOGY

影响因子： 最新[2023版] 最新五年平均 出版当年[2018版] 出版当年五年平均 出版前一年[2017版] 出版后一年[2019版]

第一作者：
第一作者机构： [1]State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [2]National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Shuai Ma,Shuhui Sun,Lingling Geng,et al.Caloric Restriction Reprograms the Single-Cell Transcriptional Landscape of Rattus Norvegicus Aging.[J].Cell.2020,180(5):984-1001.doi:10.1016/j.cell.2020.02.008.
APA：
Shuai Ma,Shuhui Sun,Lingling Geng,Moshi Song,Wei Wang...&Guang-Hui Liu.(2020).Caloric Restriction Reprograms the Single-Cell Transcriptional Landscape of Rattus Norvegicus Aging..Cell,180,(5)
MLA：
Shuai Ma,et al."Caloric Restriction Reprograms the Single-Cell Transcriptional Landscape of Rattus Norvegicus Aging.".Cell 180..5(2020):984-1001

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