Glioma is the most common type of primary central nervous system tumor. Ser/Thr protein phosphatase 5 (PP5) has been shown to regulate multiple signaling cascades that suppress growth and facilitate apoptosis in several human cancer cells. However, the role of PP5 in human gliomas remains unclear. Herein, the relationship between PP5 expression and glioma cell growth was investigated, and the therapeutic value of PP5 in glioma was further evaluated. We employed a short hairpin RNA targeting PPP5C gene to knock down PP5 expression in human glioma cell lines U251 and U373. Depletion of PPP5C via RNAi remarkably inhibited glioma cell proliferation and colony formation, and arrested cell cycle in the G0/G1 phase. Moreover, knockdown of PP5 markedly suppressed glioma cell migration, as determined by Transwell assay. Our findings suggest that PPP5C could be essential for glioma cell growth and serve as a promising therapeutic target in human gliomas.
第一作者机构：[1]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, 45 Changchun Street, Xicheng District, Beijing 100053, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, 45 Changchun Street, Xicheng District, Beijing 100053, China
推荐引用方式(GB/T 7714)：
Xinglong Zhi,Hongqi Zhang,Chuan He,et al.Serine/Threonine Protein Phosphatase-5 Accelerates Cell Growth and Migration in Human Glioma[J].CELLULAR AND MOLECULAR NEUROBIOLOGY.2015,35(5):669-677.doi:10.1007/s10571-015-0162-1.
APA：
Xinglong Zhi,Hongqi Zhang,Chuan He,Yukui Wei,Lisong Bian&Guilin Li.(2015).Serine/Threonine Protein Phosphatase-5 Accelerates Cell Growth and Migration in Human Glioma.CELLULAR AND MOLECULAR NEUROBIOLOGY,35,(5)
MLA：
Xinglong Zhi,et al."Serine/Threonine Protein Phosphatase-5 Accelerates Cell Growth and Migration in Human Glioma".CELLULAR AND MOLECULAR NEUROBIOLOGY 35..5(2015):669-677
