机构：[a]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, 45 Changchun Street, Xicheng District, Beijing 100054, China神经外科首都医科大学宣武医院[b]Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Xuhui District, Shanghai, China
Cerebral aneurysm (CA) is a life-threatening condition with a pathogenesis that remains unclear. Previous hypotheses have primarily been based on in vitro examinations of animal models. Therefore, we attempted to observe CA in living rats and to establish a multi-level evaluation system. The rat model was produced by deoxycorticosterone-acetate (DOCA; Sigma Aldrich, St. Louis, MO, USA) induced hypertension and a single injection of elastase into the basal cistern. The animals were assessed 35 days later. At the endpoint, we induced well-developed CA in 41.7% of the surviving rats. Using synchrotron radiation angiography (SRA), we observed the experimental aneurysms and their surrounding arteries dynamically in the living model. Further anatomical and histological analyses demonstrated the typical degenerative changes of the mural structure and a major infiltration of macrophages into the aneurysmal wall. In conclusion, we visualised well-developed experimental CA in living rats using SRA and demonstrated the associated degenerative histological changes and macrophage involvement; thus, we have provided an effective model for the study of dynamic multi-level changes associated with CA in a rat model. (C) 2014 Elsevier Ltd. All rights reserved.
基金：
the National Basic Research Program (973 Program) (2010CB834303)
第一作者机构：[a]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, 45 Changchun Street, Xicheng District, Beijing 100054, China
通讯作者：
通讯机构：[a]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, 45 Changchun Street, Xicheng District, Beijing 100054, China
推荐引用方式(GB/T 7714)：
Jingjing Zhao,Xiaojie Lin,Chuan He,et al.Study of cerebral aneurysms in a modified rat model: From real-time imaging to histological analysis[J].JOURNAL OF CLINICAL NEUROSCIENCE.2015,22(2):373-377.doi:10.1016/j.jocn.2014.05.044.
APA：
Jingjing Zhao,Xiaojie Lin,Chuan He,Guo-Yuan Yang&Feng Ling.(2015).Study of cerebral aneurysms in a modified rat model: From real-time imaging to histological analysis.JOURNAL OF CLINICAL NEUROSCIENCE,22,(2)
MLA：
Jingjing Zhao,et al."Study of cerebral aneurysms in a modified rat model: From real-time imaging to histological analysis".JOURNAL OF CLINICAL NEUROSCIENCE 22..2(2015):373-377
