机构：[1]Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Beijing, P.R. China首都医科大学宣武医院药物学研究室[2]Department of Neurobiology, Xuanwu Hospital of Capital Medical University, Beijing, P.R. China首都医科大学宣武医院神经生物学研究室[3]Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing, P.R. China[4]Laboratory of Chronobiology and Chronomedicine, Beijing Geriatric Medical Research Center, Beijing, P.R. China
BACKGROUND: Circadian rhythms tend to change as animals age; however, the molecular mechanisms underlying these are not yet fully understood. OBJECTIVE: To investigate whether the DNA methylation of clock genes changes with age and contributes to circadian dysfunction in aged animals. METHODS: We examined the methylation of clock promoters in the stomach, kidney, striatum, and spleen by using a methylation-specific polymerase chain reaction (MSP) assay. RESULTS: Our results show that different tissues exhibit specific patterns of clock methylation. Additionally, methylation frequency decreased significantly in older mice at the Per1 promoter in the stomach, but it was significantly increased in older mice at the Cry1, Bmal2, and Npas2 promoters in the spleen. CONCLUSION: The findings from our study suggest that DNA methylation contribute to agerelated changes in circadian rhythms in certain slave oscillators.
基金：
National Natural Science Foundation of China (No. 81071011)
Beijing key Foundation of Traditional Chinese Medicine (KJTS2011-04)
Capital health development scientific grant (2011-1001-05)
Beijing Health and Technical Personal of High-level Plan (2009-3-66)
Program for New Century Excellent Talents in University (NCET-10-0013)
New Century Talented Person Project (008-0014)
the Scientific Research Foundation for the Returned Overseas Chinese Scholars,Ministry of Education of China (45th batch)
Beijing municipal education commission new pharmaceutical group (XK100270569)
第一作者机构：[1]Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Beijing, P.R. China[3]Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing, P.R. China
通讯作者：
通讯机构：[1]Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Beijing, P.R. China[2]Department of Neurobiology, Xuanwu Hospital of Capital Medical University, Beijing, P.R. China[3]Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing, P.R. China
推荐引用方式(GB/T 7714)：
L. ZHANG,Q.-L. LIN,L. LU,et al.Tissue-specific modification of clock methylation in aging mice[J].EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES.2013,17(14):1874-1880.
APA：
L. ZHANG,Q.-L. LIN,L. LU,C.-C. YANG,Y.-L. LI...&L. LI.(2013).Tissue-specific modification of clock methylation in aging mice.EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,17,(14)
MLA：
L. ZHANG,et al."Tissue-specific modification of clock methylation in aging mice".EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 17..14(2013):1874-1880
