机构：[a]Department of Neurobiology, Xuanwu Hospital of Capital Medical University, Beijing 100053, PR China首都医科大学宣武医院神经生物学研究室[b]Department of Neurosurgery, Xuanwu Hospital of Capital Medical University, Beijing 100053, PR China神经外科首都医科大学宣武医院[c]Laboratory of Chronobiology and Chronomedicine, Beijing Geriatric Medical Research Center, Beijing 100053, PR China[d]Key Laboratory for Neurodegenerative Diseases of the Ministry of Education, Beijing 100053, PR China[e]China International Neuroscience Institute, Beijing 100053, PR China
The expression of clock genes is altered in leukocytes from patients with Parkinson's disease (PD). However, the underlying mechanisms are unknown. To determine whether abnormal CpG methylation contributes to the dysregulated expression of these genes, the methylation status of the promoters of seven major human clock genes, PER1, PER2, CRY1, CRY2, Clock, NPAS2, and BMAL1, was examined using methylation-specific PCR (MSP) and sequencing in 206 PD patients and 181 healthy controls. This analysis revealed that most clock gene promoters were devoid of methylation. Methylation was only detectable in the CRY1 and NPAS2 promoters. Interestingly, the methylation frequency of the NPAS2 promoter was significantly decreased in PD patients. These results suggest that altered promoter methylation may contribute to the abnormal expression of clock genes in PD. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
基金：
National Natural Science Foundation of China (81071011, 30940039, 30970939)
the National 973 Project Grant of China (2011CBA00408)
the Program for New Century Excellent Talents in University (NCET-10-0013)
the Science foundation of Ministry of Education of China(11120066)
第一作者机构：[a]Department of Neurobiology, Xuanwu Hospital of Capital Medical University, Beijing 100053, PR China[c]Laboratory of Chronobiology and Chronomedicine, Beijing Geriatric Medical Research Center, Beijing 100053, PR China[d]Key Laboratory for Neurodegenerative Diseases of the Ministry of Education, Beijing 100053, PR China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Neurosurgery, Xuanwu Hospital of Capital Medical University, 45 Changchun Street, Beijing 100053, PR China.[*2]Department of Neurobiology, Xuanwu Hospital of Capital Medical University, 45 Changchun Street, Beijing 100053, PR China.
推荐引用方式(GB/T 7714)：
Qingling Lin,Hui Ding,Zheng Zheng,et al.Promoter methylation analysis of seven clock genes in Parkinson's disease[J].NEUROSCIENCE LETTERS.2012,507(2):147-150.doi:10.1016/j.neulet.2011.12.007.
APA：
Qingling Lin,Hui Ding,Zheng Zheng,Zhuqin Gu,Jinghong Ma...&Yanning Cai.(2012).Promoter methylation analysis of seven clock genes in Parkinson's disease.NEUROSCIENCE LETTERS,507,(2)
MLA：
Qingling Lin,et al."Promoter methylation analysis of seven clock genes in Parkinson's disease".NEUROSCIENCE LETTERS 507..2(2012):147-150
