机构：[a]Department of Neurology and Neurobiology, Capital Medical University, Key Laboratory of Neurodegenerative Diseases (Capital Medical University), Ministry of Education, Beijing, China[b]College of Biological Sciences, University of Minnesota, St. Paul, MN, USA[c]Chinese National Human Genome Center, Beijing, China
Background: There is a growing number of clinical studies that revealed a variety of behavioral and physiological desynchronies in patients with Parkinson's disease (PD). However, these desynchronies have not been defined at the molecular level. Methods: Using real-time RT-PCR assay, we analyzed the expression profiles of two principle clock genes, PER1 and BMAL1, in total leukocytes for 12 h during the evening, overnight and morning in subjects with PD and age/sex-matched healthy controls. Results: A difference in the expression pattern of BMAL1 but not PER1 was apparent during the dark span, where the relative abundance of BMAL1 was significantly lower in PD patients versus control subjects at 21:00, 00:00 and 06:00 h. Furthermore, expression levels of BMAL1 in PD patients correlated with their United Parkinson's Disease Rating Scale score at 06:00, 09:00 h, and with Pittsburgh Sleep Quality Index score at 06:00 h. Conclusion: These results suggest that a peripheral molecular clock, as reflected in the dampened expression of the clock genes BMAL1 in total leukocytes, is altered in PD patients. In addition, the relative BMAL1 levels correlate positively with PD severity, which could provide a molecular basis to help monitor disease progression and response to investigational drugs.
基金：
National Natural Science Foundation of China (30400148, 30430280); The National 973 Project grant of China (2006CB 500701, 2006CB943703, 2007CB947704);
The national 863 Project grant of China (2006AA02A408, 2006A A02A112, 2006AA02A114); Beijing municipal science & technology commission (D07050701350703, D0705 0701350706),and financial support from Jane and Mike
Popovich.
第一作者机构：[a]Department of Neurology and Neurobiology, Capital Medical University, Key Laboratory of Neurodegenerative Diseases (Capital Medical University), Ministry of Education, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[*]Department of Neurology and Neurobiology, Xuanwu Hospital of Capital Medical University, 100053, #45 Changchun Street, Beijing, China
推荐引用方式(GB/T 7714)：
Y. Cai,S. Liu,R. B. Sothern,et al.Expression of clock genes Per1 and Bmal1 in total leukocytes in health and Parkinson's disease[J].EUROPEAN JOURNAL OF NEUROLOGY.2010,17(4):550-554.doi:10.1111/j.1468-1331.2009.02848.x.
APA：
Y. Cai,S. Liu,R. B. Sothern,S. Xu&P. Chan.(2010).Expression of clock genes Per1 and Bmal1 in total leukocytes in health and Parkinson's disease.EUROPEAN JOURNAL OF NEUROLOGY,17,(4)
MLA：
Y. Cai,et al."Expression of clock genes Per1 and Bmal1 in total leukocytes in health and Parkinson's disease".EUROPEAN JOURNAL OF NEUROLOGY 17..4(2010):550-554
