机构：[a]Cell Therapy Center, Xuanwu Hospital, Capital Medical University, Beijing, China首都医科大学宣武医院[b]Key Laboratory of Neurodegeneration, Ministry of Education, Beijing, China
Embryonic stem (ES) cells represent a valuable resource for transplantation and tissue engineering applications. For derivation of neural cells, a five-stage differentiation protocol has been widely applied, which involves the propagation of ES cells, formation of embryoid bodies (EBs), selection of neural stem cells (NSCs), expansion of NSCs, and further maturation of NSCs to neurons. During the expansion stage (the fourth stage), two types of cells with distinct morphologies normally emerge, with one type being monolayer cells and the other sphere-like aggregates growing on top of the monolayer cells. In this study, we focus on how the monolayer cells may affect different aspects of aggregate cells, which may have important implications for regenerative medicine. We find that monolayer cells can support the proliferation and decrease the apoptosis rate of sphere cells, as well as facilitate the production of Tuj1-positive cells from sphere cells. In addition, transplantation of monolayer cells into nude mice does not result in tumor formation nor affects the tumorigenicity of sphere cells, when grafted together with monolayer cells. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
基金：
High Level Talent Fund of the Beijing Healthcare System (contract grant number: 2009-2-14).
第一作者机构：[a]Cell Therapy Center, Xuanwu Hospital, Capital Medical University, Beijing, China[b]Key Laboratory of Neurodegeneration, Ministry of Education, Beijing, China
通讯作者：
通讯机构：[a]Cell Therapy Center, Xuanwu Hospital, Capital Medical University, Beijing, China
推荐引用方式(GB/T 7714)：
Qing’an Du,Yunqian Guan,Huijun Ji,et al.Effect of monolayer cells on sphere cells-Two types of cells that emerge during the neural differentiation of mouse embryonic stem cells[J].NEUROSCIENCE LETTERS.2011,504(3):285-289.doi:10.1016/j.neulet.2011.09.048.
APA：
Qing’an Du,Yunqian Guan,Huijun Ji,Zhiguo Chen&Y. Alex Zhang.(2011).Effect of monolayer cells on sphere cells-Two types of cells that emerge during the neural differentiation of mouse embryonic stem cells.NEUROSCIENCE LETTERS,504,(3)
MLA：
Qing’an Du,et al."Effect of monolayer cells on sphere cells-Two types of cells that emerge during the neural differentiation of mouse embryonic stem cells".NEUROSCIENCE LETTERS 504..3(2011):285-289
