机构：[1]Capital Med Univ, Xuanwu Hosp China, Dept Neurobiol, Beijing Inst Geriatr,Minist Educ, Beijing 100053, Peoples R China首都医科大学宣武医院[2]Capital Med Univ, Xuanwu Hosp China, Sino Japan Joint Lab Neurodegenerat Dis, Key Lab Neurodegenerat Dis,Minist Educ, Beijing 100053, Peoples R China首都医科大学宣武医院[3]Capital Med Univ, Beijing Inst Neurosci, Beijing 100053, Peoples R China[4]Tokyo Inst Psychiat, Div Psychobiol, Tokyo, Japan
Previous studies have shown that over-expression of alpha-Synuclein (alpha-Syn), a protein whose abnormality is implicated in the pathogenesis of Parkinson's disease (PD), reduces tyrosine hydroxylase (TH) expression and dopamine synthesis. To explore the possible mechanism for the regulation of TH expression by a-Syn, luciferase reporter gene carrying a -493/+27bp fragment of human TH gene (pGL3-TH520) and pcDNA carrying h alpha-Syn gene (pcDNA-h alpha-Syn) were co-transfected into T293 cells. The results showed that alpha-Syn was only detected in pcDNA-h alpha-Syn-transfected cells but not in pcDNA vector control cells. In alpha-Syn-transfected cells, the luciferase activity was dramatically reduced compared with the vector control cells. These results suggest that alpha-Syn may function as a negative regulator for TH expression by affecting the activity of TH promoter.
基金：
National High Technology Research and Development Program ("863" Program) of China [2006AA02A408]; National Basic Research Program ("973" Program) of China [2011CB504101]; National Natural Science Founution of China [30271437, 30270482, 30430280, 81071014]; Natural Science Founution of Beijing [7102076, 7022011]; Funding Project for Academic Human Resources Development in Institutions of Higher Learning under Jurisdiction of Beijing Municipality [PHR200907113]
第一作者机构：[2]Capital Med Univ, Xuanwu Hosp China, Sino Japan Joint Lab Neurodegenerat Dis, Key Lab Neurodegenerat Dis,Minist Educ, Beijing 100053, Peoples R China
通讯作者：
通讯机构：[*1]Capital Med Univ, Xuanwu Hosp China, Dept Neurobiol, Beijing Inst Geriatr,Minist Educ, 45 Changchun St, Beijing 100053, Peoples R China.
推荐引用方式(GB/T 7714)：
Li Yao-Hua,Gao Nan,Ye Yi-Wen,et al.alpha-Synuclein functions as a negative regulator for expression of tyrosine hydroxylase[J].ACTA NEUROLOGICA BELGICA.2011,111(2):130-135.
APA：
Li, Yao-Hua,Gao, Nan,Ye, Yi-Wen,Li, Xin,Yu, Shun...&Chan, Piu.(2011).alpha-Synuclein functions as a negative regulator for expression of tyrosine hydroxylase.ACTA NEUROLOGICA BELGICA,111,(2)
MLA：
Li, Yao-Hua,et al."alpha-Synuclein functions as a negative regulator for expression of tyrosine hydroxylase".ACTA NEUROLOGICA BELGICA 111..2(2011):130-135
