机构：[a]Department of Neurobiology and the Sino-Japan Joint Laboratory on Neurodegenerative Diseases, Beijing Institute of Geriatrics, Xuanwu Hospital of the Capital University of Medical Sciences, 45 Changchun Street, Beijing 100053, China老年医学科首都医科大学宣武医院[b]Department of Neural Plasticity, Tokyo Institute of Psychiatry, Tokyo 156-8585, Japan
Although the aberrant expression of alpha-synuclein (alpha-Syn) is toxic to dopaminergic neurons, little is known about the correlation between the abnormality of alpha-Syn and the expression of tyrosine hydroxylase (TH), a rate-limiting enzyme for the synthesis of dopamine neurotransmitter. In this study, the MES23.5 rat dopaminergic cell line transfected with wild-type human alpha-Syn cDNA (halpha-Syn) construct was used to investigate the association between alpha-Syn and TH. Immunocytochemical staining and Western blot for TH showed that the TH expression was greatly decreased in halpha-Syn-transfected cells. Northern blot confirmed that the TH mRNA level was also dramatically reduced. Reduction of TH protein levels did not affect the growth and proliferation of the transfected cells. No apparent cell injury or death was observed. These results suggest that an abnormal expression of alpha-Syn may inhibit TH synthesis in dopaminergic cells. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
基金：
Chinese Ministry of Science and Technology (G2000057005), National Natural Science Foundation of China (30270482 and 30271437),
and Natural Science Foundation of Beijing (7022011 and 7031002)
第一作者机构：[a]Department of Neurobiology and the Sino-Japan Joint Laboratory on Neurodegenerative Diseases, Beijing Institute of Geriatrics, Xuanwu Hospital of the Capital University of Medical Sciences, 45 Changchun Street, Beijing 100053, China
通讯作者：
通讯机构：[a]Department of Neurobiology and the Sino-Japan Joint Laboratory on Neurodegenerative Diseases, Beijing Institute of Geriatrics, Xuanwu Hospital of the Capital University of Medical Sciences, 45 Changchun Street, Beijing 100053, China
推荐引用方式(GB/T 7714)：
Shun Yu,Xiaohong Zuo,Yaohua Li,et al.Inhibition of tyrosine hydroxylase expression in alpha-synuclein-transfected dopaminergic neuronal cells[J].NEUROSCIENCE LETTERS.2004,367(1):34-39.doi:10.1016/j.neulet.2004.05.118.
APA：
Shun Yu,Xiaohong Zuo,Yaohua Li,Chen Zhang,Ming Zhou...&Piu Chan.(2004).Inhibition of tyrosine hydroxylase expression in alpha-synuclein-transfected dopaminergic neuronal cells.NEUROSCIENCE LETTERS,367,(1)
MLA：
Shun Yu,et al."Inhibition of tyrosine hydroxylase expression in alpha-synuclein-transfected dopaminergic neuronal cells".NEUROSCIENCE LETTERS 367..1(2004):34-39
