机构：[a]Department of Neurobiology and the Sino-Japan Joint Laboratory of Neurodegenerative diseases, Beijing Institute of Geriatrics, Xuanwu Hospital of Capital University of Medical Sciences, Beijing 100053, China老年医学科首都医科大学宣武医院[b]Division of Psychobiology, Tokyo Institute of Psychiatry, Tokyo 156-8585, Japan
Growing evidence suggests that oxidative stress is involved in the neuronal degeneration and can promote the aggregation of a-synuclein. However, the role of a-synuclein under physiological and pathological conditions remains poorly understood. In the present study, we examined the possible interaction between the a-synuclein and oxidative stress. In a dopaminergic cell line MES23.5, we have found that the 200 mu M H2O2 treatment induced the translocation of a-synuclein from cytoplasm to nuclei at 30 min post-treatment. The immunoactivity of a-synuclein became highly intensive in the nuclei after 2 h treatment. The protein translocated to nucleus was a 10 kDa fragment of C-terminus region of alpha-synuclein, while full-length a-synuclein remained in cytoplasm. Thioflavine-S staining suggested that the C-terminal fragment in the nuclei has no beta-sheet structures. Our present results indicated that 200 mu M H2O2 treatment induces the intranuclear accumulation of the C-terminal fragment of a-synuclein in dopaminergic neurons, whose role remains to be investigated. (c) 2006 Elsevier Inc. All rights reserved.
基金：
Grants from the National Natural Science Foundation of China (30371574, 30430280, and 30400148),
Beijing Health Bureau Basic Science Foundation No. 12, and Chinese Ministry of Science and Technology (001CB510104).
第一作者机构：[a]Department of Neurobiology and the Sino-Japan Joint Laboratory of Neurodegenerative diseases, Beijing Institute of Geriatrics, Xuanwu Hospital of Capital University of Medical Sciences, Beijing 100053, China
通讯作者：
通讯机构：[a]Department of Neurobiology and the Sino-Japan Joint Laboratory of Neurodegenerative diseases, Beijing Institute of Geriatrics, Xuanwu Hospital of Capital University of Medical Sciences, Beijing 100053, China
推荐引用方式(GB/T 7714)：
Shengli Xu,Ming Zhou,Shun Yu,et al.Oxidative stress induces nuclear translocation of C-terminus of alpha-synuclein in dopaminergic cells[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2006,342(1):330-335.doi:10.1016/j.bbrc.2006.01.148.
APA：
Shengli Xu,Ming Zhou,Shun Yu,Yanning Cai,Alex Zhang...&Piu Chan.(2006).Oxidative stress induces nuclear translocation of C-terminus of alpha-synuclein in dopaminergic cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,342,(1)
MLA：
Shengli Xu,et al."Oxidative stress induces nuclear translocation of C-terminus of alpha-synuclein in dopaminergic cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 342..1(2006):330-335
