机构：[a]Central Laboratory, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Disease of Ministry of Education, #45 Changchun Street, Xuanwu District, Beijing 100053, China首都医科大学宣武医院[b]Department of Biochemistry and Molecular Biology, Yanjing Medical College, Capital Medical University, Beijing 101300, China[c]School of Biomedical Sciences, Ulster University, Coleraine, BT52 1SA, UK
Type 2 diabetes recently has been identified as a risk factor for developing Alzheimer's disease (AD). The main reason for this appears to be insulin signaling failure in the brain. Furthermore, cholinergic neurons are particularly affected in the brains of AD patients. The aim of the present study is to investigate if insulin signaling-related proteins are co-located with cholinergic neuron in the CA1 region of hippocampus of mice, which could explain the early loss of cholinergic neurons in AD. Using immunohistochemistry, the insulin signaling-related proteins, such as insulin receptor (InsR), insulin receptor substrate-1 (IRS-1), protein kinase B (PKB, also named Akt), glycogen synthatase kinase-3bteta (GSK-3bteta) and insulin-degrading enzyme (IDE) were analysed. Choline acetyltransferase (ChAT) was selected as a marker of cholinergic neurons. in the CA1 region of hippocampus of mice, several of the insulin signaling-related proteins we bad chosen are co-located with ChAT, and most double immunoreactive positive cells were pyramidal cells. The coexistences indicated that the insulin signaling may play an important part in the activities of cholinergic neurons, and the impairment of the pathway may be important in the mechanisms that underlie neurodegeneration in AD. (C) 2008 Elsevier B.V. All rights reserved.
基金：
National Natural Science Foundation of China (30772288), Beijing Natural Science Foundation (7082043).
第一作者机构：[a]Central Laboratory, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Disease of Ministry of Education, #45 Changchun Street, Xuanwu District, Beijing 100053, China[b]Department of Biochemistry and Molecular Biology, Yanjing Medical College, Capital Medical University, Beijing 101300, China
通讯作者：
通讯机构：[a]Central Laboratory, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Disease of Ministry of Education, #45 Changchun Street, Xuanwu District, Beijing 100053, China
推荐引用方式(GB/T 7714)：
Hongjuan Wang,Rong Wang,Zhiwei Zhao,et al.Coexistences of insulin signaling-related proteins and choline acetyltransferase in neurons[J].BRAIN RESEARCH.2009,1249:237-243.doi:10.1016/j.brainres.2008.10.046.
APA：
Hongjuan Wang,Rong Wang,Zhiwei Zhao,Zhijuan Ji,Shiming Xu...&Shuli Sheng.(2009).Coexistences of insulin signaling-related proteins and choline acetyltransferase in neurons.BRAIN RESEARCH,1249,
MLA：
Hongjuan Wang,et al."Coexistences of insulin signaling-related proteins and choline acetyltransferase in neurons".BRAIN RESEARCH 1249.(2009):237-243
