机构：[1]Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 6 Tiantanxili, Beijing 100050, China重点科室诊疗科室神经外科神经外科首都医科大学附属天坛医院[2]China National Clinical Research Center for Neurological Diseases, Beijing 100050, China[3]Laborotary of Clinical Medicine Research, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China首都医科大学附属天坛医院[4]Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China[5]Beijing Key Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China[6]University of Chinese Academy of Sciences, Beijing 100049, China[7]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou 215123, China神经外科普外科苏州大学附属第二医院
As it grows in size, an intracranial aneurysm (IA) is prone to rupture. In this study, we compared two extreme groups of IAs, ruptured IAs (RIAs) smaller than 10 mm and un-ruptured IAs (UIAs) larger than 10 mm, to investigate the genes involved in the facilitation and prevention of IA rupture. The aneurismal walls of 6 smaller saccular RIAs (size smaller than 10 mm), 6 larger saccular UIAs (size larger than 10 mm) and 12 paired control arteries were obtained during surgery. The transcription profiles of these samples were studied by microarray analysis. RT-qPCR was used to confirm the expression of the genes of interest. In addition, functional group analysis of the differentially expressed genes was performed. Between smaller RIAs and larger UIAs, 101 genes and 179 genes were significantly over-expressed, respectively. In addition, functional group analysis demonstrated that the up-regulated genes in smaller RIAs mainly participated in the cellular response to metal ions and inorganic substances, while most of the up-regulated genes in larger UIAs were involved in inflammation and extracellular matrix (ECM) organization. Moreover, compared with control arteries, inflammation was up-regulated and muscle-related biological processes were down-regulated in both smaller RIAs and larger UIAs. The genes involved in the cellular response to metal ions and inorganic substances may facilitate the rupture of IAs. In addition, the healing process, involving inflammation and ECM organization, may protect IAs from rupture.
基金：
This research was supported by the cooperative project between
The Science and Technology Ministry of China and Canada titled
BResearch on the genetics and pathogenesis of intracranial aneurysm and
arteriovenous malformation^ (No. 2010 dfb30850).
第一作者机构：[1]Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 6 Tiantanxili, Beijing 100050, China[2]China National Clinical Research Center for Neurological Diseases, Beijing 100050, China
通讯作者：
通讯机构：[1]Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 6 Tiantanxili, Beijing 100050, China[2]China National Clinical Research Center for Neurological Diseases, Beijing 100050, China
推荐引用方式(GB/T 7714)：
Hao Li,Haowen Li,Haiyan Yue,et al.Comparison between smaller ruptured intracranial aneurysm and larger un-ruptured intracranial aneurysm: gene expression profile analysis[J].NEUROSURGICAL REVIEW.2017,40(3):419-425.doi:10.1007/s10143-016-0799-3.
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