Systematically profiling the expression of eIF3 subunits in glioma reveals the expression of eIF3i has prognostic value in IDH-mutant lower grade glioma
机构：[1]Department of Molecular Neuropathology, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, No. 119 Nan Si Huan Xi Road, Fengtai District, Beijing 100160, China.研究所北京市神经外科研究所首都医科大学附属天坛医院[2]Department of Clinical Laboratory, Beijing Chao?Yang Hospital, Capital Medical University, Beijing 100020, China.[3]Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 119 Nan Si Huan Xi Road, Fengtai District, Beijing 100160, China.重点科室诊疗科室神经外科神经外科首都医科大学附属天坛医院[4]China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing 100160, China.重点科室诊疗科室神经病学中心神经病学中心首都医科大学附属天坛医院[5]Xiang Fen Centers for Disease Control and Prevention, Xiangfen 041500, Shanxi, China.[6]Chinese Glioma Genome Atlas Network (CGGA), Beijing, China.
BackgroundAbnormal expression of the eukaryotic initiation factor 3 (eIF3) subunits plays critical roles in tumorigenesis and progression, and also has potential prognostic value in cancers. However, the expression and clinical implications of eIF3 subunits in glioma remain unknown.MethodsExpression data of eIF3 for patients with gliomas were obtained from the Chinese Glioma Genome Atlas (CGGA) (n=272) and The Cancer Genome Atlas (TCGA) (n=595). Cox regression, the receiver operating characteristic (ROC) curves and Kaplan-Meier analysis were used to study the prognostic value. Gene oncology (GO) and gene set enrichment analysis (GSEA) were utilized for functional prediction.ResultsIn both the CGGA and TCGA datasets, the expression levels of eIF3d, eIF3e, eIF3f, eIF3h and eIF3l highly were associated with the IDH mutant status of gliomas. The expression of eIF3b, eIF3i, eIF3k and eIF3m was increased with the tumor grade, and was associated with poorer overall survival [All Hazard ratio (HR)>1 and P<0.05]. By contrast, the expression of eIF3a and eIF3l was decreased in higher grade gliomas and was associated with better overall survival (Both HR<1 and P<0.05). Importantly, the expression of eIF3i (located on chromosome 1p) and eIF3k (Located on chromosome 19q) were the two highest risk factors in both the CGGA [eIF3i HR=2.068 (1.425-3.000); eIF3k HR=1.737 (1.166-2.588)] and TCGA [eIF3i HR=1.841 (1.642-2.064); eIF3k HR=1.521 (1.340-1.726)] databases. Among eIF3i, eIF3k alone or in combination, the expression of eIF3i was the more robust in stratifying the survival of glioma in various pathological subgroups. The expression of eIF3i was an independent prognostic factor in IDH-mutant lower grade glioma (LGG) and could also predict the 1p/19q codeletion status of IDH-mutant LGG. Finally, GO and GSEA analysis showed that the elevated expression of eIF3i was significantly correlated with the biological processes of cell proliferation, mRNA processing, translation, T cell receptor signaling, NF-B signaling and others.ConclusionsOur study reveals the expression alterations during glioma progression, and highlights the prognostic value of eIF3i in IDH-mutant LGG.
基金：
National Key Research and Development Program of China [2018YFC0115604]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81773208]; National Natural Science Foundation of China (NSFC)/Research Grants Council (RGC) Joint Research Scheme [81761168038]; Beijing Municipal Administration of Hospitals' Mission Plan [SML20180501, 2018.03-2022.02]
第一作者机构：[1]Department of Molecular Neuropathology, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, No. 119 Nan Si Huan Xi Road, Fengtai District, Beijing 100160, China.[4]China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing 100160, China.[6]Chinese Glioma Genome Atlas Network (CGGA), Beijing, China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Molecular Neuropathology, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, No. 119 Nan Si Huan Xi Road, Fengtai District, Beijing 100160, China.[3]Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 119 Nan Si Huan Xi Road, Fengtai District, Beijing 100160, China.[4]China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing 100160, China.[6]Chinese Glioma Genome Atlas Network (CGGA), Beijing, China.
推荐引用方式(GB/T 7714)：
Chai Rui-Chao,Wang Ning,Chang Yu-Zhou,et al.Systematically profiling the expression of eIF3 subunits in glioma reveals the expression of eIF3i has prognostic value in IDH-mutant lower grade glioma[J].CANCER CELL INTERNATIONAL.2019,19(1):-.doi:10.1186/s12935-019-0867-1.
APA：
Chai, Rui-Chao,Wang, Ning,Chang, Yu-Zhou,Zhang, Ke-Nan,Li, Jing-Jun...&Wang, Yong-Zhi.(2019).Systematically profiling the expression of eIF3 subunits in glioma reveals the expression of eIF3i has prognostic value in IDH-mutant lower grade glioma.CANCER CELL INTERNATIONAL,19,(1)
MLA：
Chai, Rui-Chao,et al."Systematically profiling the expression of eIF3 subunits in glioma reveals the expression of eIF3i has prognostic value in IDH-mutant lower grade glioma".CANCER CELL INTERNATIONAL 19..1(2019):-
