Cytoprotective effect of atmospheric pressure helium plasma on oxygen and glucose deprivation-induced cell death in H9C2 cardiac myoblasts and primary neonatal rat cardiomyocytes
机构：[1]Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, People’s Republic of China研究所北京市神经外科研究所首都医科大学附属天坛医院[2]School of Physics, Beijing Institute of Technology, Beijing 100081, People’s Republic of China[3]Institute for Health and Biomedical Innovation and School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, Brisbane, Queensland 4000, Australia[4]CSIRO-QUT Joint Sustainable Processes and Devices Laboratory, PO Box 218, Bradfield Road, Lindfield, New South Wales 2070, Australia
Atmospheric pressure plasma jet (APPJ) has shown excellent potential prospects in biomedical applications, based on the production of reactive oxygen species and reactive nitrogen species (RNS) from APPJ emissions. The current research focused on the protective effect of APPJ on oxygen and glucose deprivation (OGD)-induced cell death in both the H9C2 cardiac myoblast cell line, a frequently used cardiac cell line in cardioprotective studies, and primary neonatal rat cardiomyocytes (NRCMs). Cells were treated with APPJ for different durations, cultured for 6h and then subjected to OGD for 18h before their use in assays. We found that APPJ treatment could maintain H9C2 cell viability and reduce cell apoptosis in a dose-dependent manner in cells subjected to the OGD conditions. To confirm the cardioprotective effect of APPJ on primary NRCM, we first identified the 'safe dose' of APPJ treatment by evaluating the cytotoxicity of APPJ on primary NRCMs in normal culture conditions. Under the 'safe dose' of APPJ treatment, we also found that the APPJ treatment could maintain NRCM viability under OGD conditions and reduce CK-MB and cTnI release from cardiomyocytes. Further studies revealed that the cytoprotective effect of APPJ may be related to NO production induced by APPJ treatment. Our results gave the first evidence of the cardiotoxicity and cytoprotective effect of APPJ on cardiomyocytes against OGD injury, and furthermore, contributed to new insights into the potential medical applications of plasma in cardiovascular diseases.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [51707012, 11475019]
第一作者机构：[1]Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, People’s Republic of China[2]School of Physics, Beijing Institute of Technology, Beijing 100081, People’s Republic of China
推荐引用方式(GB/T 7714)：
Yan Xu,Zhang Chenyang,Ouyang Jiting,et al.Cytoprotective effect of atmospheric pressure helium plasma on oxygen and glucose deprivation-induced cell death in H9C2 cardiac myoblasts and primary neonatal rat cardiomyocytes[J].JOURNAL OF PHYSICS D-APPLIED PHYSICS.2019,52(13):-.doi:10.1088/1361-6463/aafe9a.
APA：
Yan, Xu,Zhang, Chenyang,Ouyang, Jiting,Meng, Zhaozhong,Shi, Zhongfang...&Ostrikov, Kostya (Ken).(2019).Cytoprotective effect of atmospheric pressure helium plasma on oxygen and glucose deprivation-induced cell death in H9C2 cardiac myoblasts and primary neonatal rat cardiomyocytes.JOURNAL OF PHYSICS D-APPLIED PHYSICS,52,(13)
MLA：
Yan, Xu,et al."Cytoprotective effect of atmospheric pressure helium plasma on oxygen and glucose deprivation-induced cell death in H9C2 cardiac myoblasts and primary neonatal rat cardiomyocytes".JOURNAL OF PHYSICS D-APPLIED PHYSICS 52..13(2019):-
