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Simultaneous activation of impaired autophagy and the mammalian target of rapamycin pathway in oral squamous cell carcinoma

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资源类型：

收录情况： ◇ SCIE

作者：
Xubin Yin[1,2] * ; Liang Hu[1] ; Xiaoyu Feng[1] ; Hongyun Wang[3] ; Chunmei Zhang[1] ; Hao Wang[2] ; Songlin Wang[1,4,*1] ;
机构： [1]Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China [2]Beijing Tian Tan Hospital, Capital Medical University, Beijing, China [3]Beijing Neurosurgical Institute, Capital Medical University, Beijing, China [4]Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Beijing, China
出处：
DOI： 10.1111/jop.12884
ISSN：

关键词： autophagy light chain 3 mammalian target of rapamycin oral squamous cell carcinoma p62

摘要：
Background: The aim of this study was to conduct a comparative evaluation of the expression levels of autophagy markers and proteins of the mammalian target of rapamycin (mTOR) pathway in normal, margin and tumour tissues of patients with oral squamous cell carcinoma (OSCC). Materials and Methods: Three regional specimens, including normal, margin and tumour tissues, were collected from 26 patients with OSCC. Western blotting, immunohistochemistry and immunofluorescence staining were performed to detect mTOR, eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1), p70 ribosomal S6 protein kinase (p70S6K) and the corresponding phosphorylated proteins, as well as the light chain 3 (LC3) and sequestosome-1 (SQSTM1, also known as p62) autophagy indicators. Results: LC3-II, p62, mTOR, phospho-mTOR, 4E-BP1 and phospho-4E-BP1 were highly expressed in the margin and tumour groups. There were positive correlations between mTOR/phospho-mTOR, mTOR/4E-BP1, mTOR/phospho-4E-BP1, mTOR/p70S6K, LC3-II/p62, LC3-II/p70S6K, p62/4E-BP1 and p62/phospho-4E-BP1 in normal group, while LC3-II/p62, LC3-II/mTOR, LC3-II/4E-BP1, LC3-II/phospho-4E-BP1, phospho-4E-BP1/mTOR, phospho-4E-BP1/4E-BP1 and p62/4E-BP1 showed positive relationships in margin group; however, in tumour group, only mTOR/phospho-mTOR, 4E-BP1/phospho-4E-BP1 and phospho-mTOR/p70S6K showed positive correlations. Conclusion: The study suggests that autophagy is impaired in patients with OSCC and impaired autophagy and the mTOR pathway are simultaneously activated in OSCC cells. © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

基金：
Funding information This study was supported by the National Natural Science Foundation of China (91649124) and Beijing Municipality Government (Beijing Scholar Program-PXM2018_014226_000021, PXM2017_014226_000023 and PXM2018_193312_000006_0028S643_FCG) (S. Wang). We are grateful to Hua Gao and Lei Gong for their technical support on immunohistochemistry and Western blotting, respectively.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类 | 4 区 医学
小类 | 3 区 牙科与口腔外科 3 区 病理学
最新[2023]版：
大类 | 3 区 医学
小类 | 3 区 牙科与口腔外科 3 区 病理学
JCR分区：
出版当年[2017]版：
Q1 DENTISTRY, ORAL SURGERY & MEDICINE Q2 PATHOLOGY
最新[2023]版：
Q1 DENTISTRY, ORAL SURGERY & MEDICINE Q2 PATHOLOGY

影响因子： 最新[2023版] 最新五年平均 出版当年[2017版] 出版当年五年平均 出版前一年[2016版] 出版后一年[2018版]

第一作者：
第一作者机构： [1]Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China [2]Beijing Tian Tan Hospital, Capital Medical University, Beijing, China
通讯作者：
通讯机构： [1]Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China [4]Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Beijing, China [*1]Capital Medical University School of Stomatology, Beijing 100050, China.
推荐引用方式(GB/T 7714)：
Xubin Yin,Liang Hu,Xiaoyu Feng,et al.Simultaneous activation of impaired autophagy and the mammalian target of rapamycin pathway in oral squamous cell carcinoma[J].JOURNAL OF ORAL PATHOLOGY & MEDICINE.2019,48(8):705-711.doi:10.1111/jop.12884.
APA：
Xubin Yin,Liang Hu,Xiaoyu Feng,Hongyun Wang,Chunmei Zhang...&Songlin Wang.(2019).Simultaneous activation of impaired autophagy and the mammalian target of rapamycin pathway in oral squamous cell carcinoma.JOURNAL OF ORAL PATHOLOGY & MEDICINE,48,(8)
MLA：
Xubin Yin,et al."Simultaneous activation of impaired autophagy and the mammalian target of rapamycin pathway in oral squamous cell carcinoma".JOURNAL OF ORAL PATHOLOGY & MEDICINE 48..8(2019):705-711

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