机构：[1]Departments of Neurosurgery,Stanford University, CA[2]Biological Sciences,Stanford University, CA[3]Stanford Stroke Center, Stanford University, CA[4]Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China[5]Department of Neurosurgery, Xuanwu Hospital, Capital Medical School, Beijing, China神经外科首都医科大学宣武医院
Background and Purpose-Whether the mammalian target of rapamycin (mTOR) pathway is protective against brain injury from stroke or is detrimental is controversial, and whether it is involved in the protective effects of ischemic postconditioning (IPC) against stroke is unreported. Our study focuses on the protective role of mTOR against neuronal injury after stroke with and without IPC. Methods-We used both an in vitro oxygen-glucose deprivation model with a mixed neuronal culture and hypoxic postconditioning, as well as an in vivo stroke model with IPC. Rapamycin, a specific pharmacological inhibitor of mTOR, and mTOR short hairpin RNA lentiviral vectors were used to inhibit mTOR activity. A lentiviral vector expressing S6K1, a downstream molecule of mTOR, was used to confirm the protective effects of mTOR. Infarct sizes were measured and protein levels were examined by Western blot. Results-We report that stroke resulted in reduced levels of phosphorylated proteins in the mTOR pathway, including S6K1, S6, and 4EBP1, and that IPC increased these proteins. mTOR inhibition, both by the mTOR inhibitor rapamycin and by mTOR short hairpin RNA, worsened ischemic outcomes in vitro and in vivo and abolished the protective effects of hypoxic postconditioning and IPC on neuronal death in vitro and brain injury size in vivo. Overexpression of S6K1 mediated by lentiviral vectors significantly attenuated brain infarction. Conclusions-mTOR plays a crucial protective role in brain damage after stroke and contributes to the protective effects of IPC.
基金：
American Heart Association grant-inaid (10GRNT4200024 and 1R01NS064136-01).
第一作者机构：[1]Departments of Neurosurgery,Stanford University, CA[4]Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China
通讯作者：
通讯机构：[5]Department of Neurosurgery, Xuanwu Hospital, Capital Medical School, Beijing, China[*1]Department of Neurosurgery, Stanford University School of Medicine, 1201 Welch Rd, Room 306, MSLS Bldg, Stanford, CA 94305-5327,
推荐引用方式(GB/T 7714)：
Rong Xie ,Peng Wang ,Michelle Cheng ,et al.Mammalian Target of Rapamycin Cell Signaling Pathway Contributes to the Protective Effects of Ischemic Postconditioning Against Stroke[J].STROKE.2014,45(9):2769-2776.doi:10.1161/STROKEAHA.114.005406.
APA：
Rong Xie,,Peng Wang,,Michelle Cheng,,Robert Sapolsky,,Xunming Ji,&Heng Zhao,.(2014).Mammalian Target of Rapamycin Cell Signaling Pathway Contributes to the Protective Effects of Ischemic Postconditioning Against Stroke.STROKE,45,(9)
MLA：
Rong Xie,,et al."Mammalian Target of Rapamycin Cell Signaling Pathway Contributes to the Protective Effects of Ischemic Postconditioning Against Stroke".STROKE 45..9(2014):2769-2776
医学