机构：[1]Department of Neurosurgery, The First Affiliated Hospital of Baotou Medical College, Baotou, Inner Mongolia 014010[2]Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012[3]Department of Neurosurgery, Fuxing Hospital, Capital Medical University, Beijing 100038[4]The Second Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515[5]Department of Neurosurgery, Brain Tumor Research Center, Beijing Neurosurgical Institute, Beijing Tiantan Hospital Affiliated with The Capital Medical University, Beijing 100050, P.R. China研究所北京市神经外科研究所首都医科大学附属天坛医院
Expression of the long non-coding RNA taurine-upregulated gene 1 (TUG1) is associated with various aggressive tumors. The present study aimed to investigate the biological function of TUG1 in regulating apoptosis, proliferation, invasion and cell cycle distribution in human glioma U251 cells. Lentivirus-mediated TUG1-specific microRNA was transfected into U251 cells to abrogate the expression of TUG1. Flow cytometry analysis was used to examine the cell cycle distribution and apoptosis of U251 cells. Cellular proliferation was examined using Cell Counting Kit-8 (CCK-8) assays and invasion was examined by Transwell assays. The apoptotic rate of cells in the TUG1-knockdown group was significantly higher than in the negative control (NC) group (11.58 vs. 9.14%, P<0.01). CCK-8 assay data demonstrated that the proliferative ability of cells within the TUG1-knockdown group was lower compared with that of the NC group. A Transwell invasion assay was performed, which revealed that the number of invaded cells from the TUG1-knockdown group was the less compared with that of the NC group. In addition, the G(0)/G(1) phase population was significantly increased within the treated group (44.85 vs. 38.45%, P<0.01), as measured by flow cytometry. The present study demonstrated that the downregulation of TUG1 may inhibit proliferation and invasion, and promote glioma U251 cell apoptosis. In addition, knockdown of TUG1 may have an effect on cell cycle arrest. The data presented in the current study indicated that TUG1 may be a novel therapeutic target for glioma.
基金：
Inner Mongolia Natural Science Foundation [2015MS0859]; Young Scientists Foundation of Fu Xing Hospital of Capital Medical University [2014YQ]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81372354, 81302186, 81271563]
第一作者机构：[1]Department of Neurosurgery, The First Affiliated Hospital of Baotou Medical College, Baotou, Inner Mongolia 014010[2]Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012
通讯作者：
通讯机构：[2]Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012[3]Department of Neurosurgery, Fuxing Hospital, Capital Medical University, Beijing 100038[*1]Department of Neurosurgery, Qilu Hospital of Shandong University, 107 Wen Hua Xi lu, Lixia, Jinan, Shandong 250012, P.R. China
推荐引用方式(GB/T 7714)：
ZHIJUN ZHAO,BIN WANG,JUNHAI HAO,et al.Downregulation of the long non-coding RNA taurine-upregulated gene 1 inhibits glioma cell proliferation and invasion and promotes apoptosis[J].ONCOLOGY LETTERS.2018,15(3):4026-4032.doi:10.3892/ol.2018.7784.
APA：
ZHIJUN ZHAO,BIN WANG,JUNHAI HAO,WEITAO MAN,YONGKAI CHANG...&JUN YANG.(2018).Downregulation of the long non-coding RNA taurine-upregulated gene 1 inhibits glioma cell proliferation and invasion and promotes apoptosis.ONCOLOGY LETTERS,15,(3)
MLA：
ZHIJUN ZHAO,et al."Downregulation of the long non-coding RNA taurine-upregulated gene 1 inhibits glioma cell proliferation and invasion and promotes apoptosis".ONCOLOGY LETTERS 15..3(2018):4026-4032
