Our previous miRNAs profiling study showed that miR-27b was up-regulated in glioma cells compared with H4 low grade astrocytoma cells. However, the main function of miR-27b in glioma in not known yet. The aim of this study was to investigate the expression and function of miR-27b in the pathogenesis of glioma. Real-time PCR showed that miR-27b was up-regulated in glioma samples and glioma cells. Downregulation of miR-27b triggered growth inhibition, induced apoptosis and inhibited invasion in glioma cells. Furthermore, TOPflash luciferase activity was decreased significantly, while FOPflash luciferase did not change significantly. In addition, Western blot assay showed that STAT3, c-myc and cyclin D1 were knocked down after treatment with miR-27b inhibitor. These findings suggest that aberrantly up-regulated miR-27b may be one of the critical factors that contribute to malignancy in human gliomas.
基金：
China National Natural Scientific FundNational Natural Science Foundation of China [30971136, 81001128]; Program for New Century Excellent Talents in UniversityProgram for New Century Excellent Talents in University (NCET) [NCET-07-0615]; Tianjin Science and Technology Committee [09JCZDJC17600]
通讯机构：[1]Tianjin Med Univ, Gen Hosp, Tianjin Neurol Inst, Lab Neurooncol,Dept Neurosurg, Tianjin 300060, Peoples R China;[2]Minist Educ, Key Lab Neurotrauma Variat & Regenerat, Tianjin 300060, Peoples R China;[3]Tianjin Municipal Govt, Tianjin 300060, Peoples R China;[6]Tianjin Neurol Inst, Lab Neurooncol, 152 Ahshan Rd, Tianjin 300052, Peoples R China
推荐引用方式(GB/T 7714)：
Chen Lingchao,Li Huibing,Han Lei,et al.Expression and function of miR-27b in human glioma[J].ONCOLOGY REPORTS.2011,26(6):1617-1621.doi:10.3892/or.2011.1458.
APA：
Chen, Lingchao,Li, Huibing,Han, Lei,Zhang, Kailiang,Wang, Guangxiu...&Kang, Chunsheng.(2011).Expression and function of miR-27b in human glioma.ONCOLOGY REPORTS,26,(6)
MLA：
Chen, Lingchao,et al."Expression and function of miR-27b in human glioma".ONCOLOGY REPORTS 26..6(2011):1617-1621
