The present study was to investigate effects of Silica nanoparticles (SiNPs) on nervous system and explore potential mechanisms in human neuroblastoma cells (SH-SY5Y). Cytotoxicity was detected by cell viability and Lactate dehydrogenase (LDH) release. Flow cytometry analysis was applied to assess mitochondrial membrane potential (MMP) loss, intracellular Ca2+ and apoptosis. To clarify the mechanism of SiNPs-induced apoptosis, intrinsic apoptosis-related proteins were detected. Our results showed that SiNPs caused cytotoxicity, cell membrane damage and Ca2+ increase in a dose-dependent manner in SH-SY5Y cells. Both the mitochondrial membrane potential (MMP) loss and potential mitochondria damage resulted in Cyt C release to the cytoplasm. The elevated Cyt C and Apaf1 further triggered intrinsic apoptosis via executive molecular caspase-9 and caspase-3. The present study confirmed that SiNPs induced intrinsic apoptosis in neuroblastoma SH-SY5Y cells via CytC/Apaf-1 pathway and provided a better understanding of the potential toxicity induced by SiNPs on human neurocyte. (C) 2017 Elsevier B.V. All rights reserved.
基金:
Beijing Key Laboratory of Environmental Toxicology, Capital Medical University [2015HJDL04]; Undergraduate Innovative and Pioneering Training Program, Jilin University [2013B72323]
第一作者机构:[1]Jilin Univ, Sch Publ Hlth, Changchun 130021, Peoples R China;
通讯作者:
通讯机构:[1]Jilin Univ, Sch Publ Hlth, Changchun 130021, Peoples R China;[3]Capital Med Univ, Sch Publ Hlth, Beijing 100069, Peoples R China;[4]Capital Med Univ, Beijing Key Lab Environm Toxicol, Beijing, Peoples R China;
推荐引用方式(GB/T 7714):
Yang Yanyan,Yu Yongbo,Wang Jiahui,et al.Silica nanoparticles induced intrinsic apoptosis in neuroblastoma SH-SY5Y cells via CytC/Apaf-1 pathway[J].ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY.2017,52:161-169.doi:10.1016/j.etap.2017.01.010.
APA:
Yang, Yanyan,Yu, Yongbo,Wang, Jiahui,Li, Yanbo,Li, Yang...&Sun, Zhiwei.(2017).Silica nanoparticles induced intrinsic apoptosis in neuroblastoma SH-SY5Y cells via CytC/Apaf-1 pathway.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,52,
MLA:
Yang, Yanyan,et al."Silica nanoparticles induced intrinsic apoptosis in neuroblastoma SH-SY5Y cells via CytC/Apaf-1 pathway".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 52.(2017):161-169