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Silica nanoparticles induced intrinsic apoptosis in neuroblastoma SH-SY5Y cells via CytC/Apaf-1 pathway

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机构: [1]Jilin Univ, Sch Publ Hlth, Changchun 130021, Peoples R China; [2]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Beijing Key Lab Pediat Dis Otolaryngol Head & Nec, Beijing, Peoples R China; [3]Capital Med Univ, Sch Publ Hlth, Beijing 100069, Peoples R China; [4]Capital Med Univ, Beijing Key Lab Environm Toxicol, Beijing, Peoples R China; [5]Jilin Univ, Coll Clin Med, Norman Bethune Hlth Sci Ctr, Changchun, Peoples R China
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关键词: Silica nanoparticles SH-SY5Y Mitochondrial membrane potential (MMP) Intrinsic apoptosis

摘要:
The present study was to investigate effects of Silica nanoparticles (SiNPs) on nervous system and explore potential mechanisms in human neuroblastoma cells (SH-SY5Y). Cytotoxicity was detected by cell viability and Lactate dehydrogenase (LDH) release. Flow cytometry analysis was applied to assess mitochondrial membrane potential (MMP) loss, intracellular Ca2+ and apoptosis. To clarify the mechanism of SiNPs-induced apoptosis, intrinsic apoptosis-related proteins were detected. Our results showed that SiNPs caused cytotoxicity, cell membrane damage and Ca2+ increase in a dose-dependent manner in SH-SY5Y cells. Both the mitochondrial membrane potential (MMP) loss and potential mitochondria damage resulted in Cyt C release to the cytoplasm. The elevated Cyt C and Apaf1 further triggered intrinsic apoptosis via executive molecular caspase-9 and caspase-3. The present study confirmed that SiNPs induced intrinsic apoptosis in neuroblastoma SH-SY5Y cells via CytC/Apaf-1 pathway and provided a better understanding of the potential toxicity induced by SiNPs on human neurocyte. (C) 2017 Elsevier B.V. All rights reserved.

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出版当年[2016]版:
大类 | 4 区 医学
小类 | 3 区 环境科学 4 区 药学 4 区 毒理学
最新[2023]版:
大类 | 3 区 环境科学与生态学
小类 | 2 区 毒理学 3 区 环境科学 3 区 药学
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出版当年[2015]版:
Q2 ENVIRONMENTAL SCIENCES Q3 PHARMACOLOGY & PHARMACY Q3 TOXICOLOGY
最新[2023]版:
Q1 PHARMACOLOGY & PHARMACY Q1 TOXICOLOGY Q2 ENVIRONMENTAL SCIENCES

影响因子: 最新[2023版] 最新五年平均 出版当年[2015版] 出版当年五年平均 出版前一年[2014版] 出版后一年[2016版]

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第一作者机构: [1]Jilin Univ, Sch Publ Hlth, Changchun 130021, Peoples R China;
通讯作者:
通讯机构: [1]Jilin Univ, Sch Publ Hlth, Changchun 130021, Peoples R China; [3]Capital Med Univ, Sch Publ Hlth, Beijing 100069, Peoples R China; [4]Capital Med Univ, Beijing Key Lab Environm Toxicol, Beijing, Peoples R China;
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