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Metagenomic analysis of viral genetic diversity in respiratory samples from children with severe acute respiratory infection in China

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机构: [1]Minist Hlth, Key Lab Med Virol, Beijing, Peoples R China; [2]Natl Inst Viral Dis Control & Prevent, Beijing, Peoples R China; [3]Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China; [4]Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Beijing, Peoples R China; [5]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Key Lab Major Dis Children,Minist Educ, Beijing, Peoples R China; [6]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Natl Key Discipline Paediat,Minist Educ, Beijing, Peoples R China; [7]China CDC, Natl Inst Viral Dis Control & Prevent, Minist Hlth, Key Lab Med Virol, 155 Changbai Rd, Beijing 102206, Peoples R China
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关键词: Children metagenomics next-generation sequencing severe acute respiratory tract infection virome

摘要:
Severe acute respiratory infection (SARI) in children is thought to be mainly caused by infection with various viruses, some of which have been well characterized; however, analyses of respiratory tract viromes among children with SARI versus those without are limited. In this study, nasopharyngeal swabs from children with and without SARI (135 versus 15) were collected in China between 2008 and 2010 and subjected to multiplex metagenomic analyses using a next-generation sequencing platform. The results show that members of the Paramyxoviridae, Coronaviridae, Parvoviridae, Orthomyxoviridae, Picornaviridae, Anelloviridae and Adenoviridae families represented the most abundant species identified (>50% genome coverage) in the respiratory tracts of children with SARI. The viral population found in the respiratory tracts of children without SARI was less diverse and mainly dominated by the Anelloviridae family with only a small proportion of common epidemic respiratory viruses. Several almost complete viral genomes were assembled, and the genetic diversity was determined among several samples based on next-generation sequencing. This research provides comprehensive mapping of the viromes of children with SARI and indicates high heterogeneity of known viruses present in the childhood respiratory tract, which may benefit the detection and prevention of respiratory disease. (C) 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

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出版当年[2015]版:
大类 | 2 区 医学
小类 | 2 区 传染病学 2 区 微生物学
最新[2023]版:
大类 | 1 区 医学
小类 | 1 区 传染病学 1 区 微生物学
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出版当年[2014]版:
Q1 INFECTIOUS DISEASES Q1 MICROBIOLOGY
最新[2023]版:
Q1 INFECTIOUS DISEASES Q1 MICROBIOLOGY

影响因子: 最新[2023版] 最新五年平均 出版当年[2014版] 出版当年五年平均 出版前一年[2013版] 出版后一年[2015版]

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第一作者机构: [1]Minist Hlth, Key Lab Med Virol, Beijing, Peoples R China; [2]Natl Inst Viral Dis Control & Prevent, Beijing, Peoples R China;
通讯作者:
通讯机构: [1]Minist Hlth, Key Lab Med Virol, Beijing, Peoples R China; [2]Natl Inst Viral Dis Control & Prevent, Beijing, Peoples R China; [7]China CDC, Natl Inst Viral Dis Control & Prevent, Minist Hlth, Key Lab Med Virol, 155 Changbai Rd, Beijing 102206, Peoples R China
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