Objective: To discuss the effect of BRMS1 on the proliferation, migration and adhesion of mouse forestomach carcinoma. Methods: The constructed pCMV-myc-BRMS1 recombinant plasmid and blank plasmid were transfected into mouse forestomach carcinoma. MTT method was employed to measure the activity of gastric cancer cell; the scratch assay and Transwell assay to measure the migration and invasion of gastric cancer cell; the adhesion assay to measure the adhesion of gastric cancer cell; while the Western blot assay to measure the expression of The NF-kappa B signal pathway, downstream matrix metalloproteinase (MMP)-2, MMP-9 and osteopontin and E-cadherin in the gastric cancer cell. Besides, the transplanted animal model of gastric cancer in mice was constructed to measure the size of tumor xenograft. Results: Results of MTT assay showed that, compared with the empty vector control group, the activity of gastric cancer cell was not affected in the BRMS1 transfection group. The improved expression of BRMS1 could inhibit the adhesion, migration and invasion of gastric cancer cell (P<0.01). Besides, compared with the empty vector control group, the phosphorylation of NF-kappa B p65 and I kappa B was reduced in the BRMS1 transfection group, with the decreased expression of MMP-2, MMP-9 and osteopontin and the increased expression of E-cadherin (P<0.01). Results of animal experiment also showed that the expression of BRMS1 did not affect the transplanted tumor. Conclusions: The expression of BRMS1 can significantly inhibit the adhesion, migration, invasion and metastasis of mouse forestomach carcinoma gastric cancer cell, which is related to The NF-kappa B signal pathway.
基金：
National Science Fund for Distinguished Young ScholarsNational Natural Science Foundation of ChinaNational Science Fund for Distinguished Young Scholars [0602150028]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2014]版：
大类|4 区医学
小类|4 区公共卫生、环境卫生与职业卫生4 区热带医学
最新[2025]版：
大类|4 区医学
小类|4 区公共卫生、环境卫生与职业卫生4 区热带医学
JCR分区：
出版当年[2013]版：
Q2TROPICAL MEDICINEQ4PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
最新[2023]版：
Q2TROPICAL MEDICINEQ3PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
第一作者机构：[1]Capital Med Univ, Beijing Tiantan Hosp, Dept Digest Syst, Beijing 100050, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Tiantan Hosp, Dept Digest Syst, Beijing 100050, Peoples R China;
推荐引用方式(GB/T 7714)：
Guo Xiu-Li,Wang Ya-Jie,Cui Pei-Lin,et al.Effect of BRMS1 expression on proliferation, migration and adhesion of mouse forestomach carcinoma[J].ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE.2015,8(9):712-717.doi:10.1016/j.apjtm.2015.07.020.
APA：
Guo, Xiu-Li,Wang, Ya-Jie,Cui, Pei-Lin,Wang, Yan-Bin,Liang, Pi-Xia...&Xu, You-Qing.(2015).Effect of BRMS1 expression on proliferation, migration and adhesion of mouse forestomach carcinoma.ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE,8,(9)
MLA：
Guo, Xiu-Li,et al."Effect of BRMS1 expression on proliferation, migration and adhesion of mouse forestomach carcinoma".ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE 8..9(2015):712-717
