机构：[1]Capital Med Univ, Sch Tradit Chinese Med, Beijing 100069, Peoples R China;[2]Capital Med Univ, Beijing Tian Tan Hosp, Beijing 100050, Peoples R China;[3]Beijing Inst Drug Control, Beijing 100035, Peoples R China
Background: T helper (Th) 17 and regulatory T (Treg) cells play a critical role in the pathogenesis of multiple sclerosis (MS) disease. Bu Shen Yi Sui Capsule (BSYSC), a traditional Chinese medicine formula, has been used clinically for the treatment of MS patients in China. Methods: To evaluate the neuroprotective effects and the underlying mechanisms of BSYSC on MS, experimental autoimmune encephalomyelitis (EAE) model in C57BL/6 mice was induced with myelin oligodendrocyte glycoprotein (MOG) 35-55. Th17 and Treg cells and the related cytokines were detected by flow cytometry, ELISA, real-time quantitative reverse transcription PCR, western blot and immunohistochemistry. Results: We found that BSYSC improved neurological function, reduced inflammatory cell infiltration and damage to the axons and myelin in the brain and spinal cord. BSYSC down-regulated markedly the ratio of CD4 + IL-17+/CD4 + CD25 + FoxP3+T cells in the spleen, decreased the cytokines of IL-17A, IL-6, IL-23, TGF-beta1 in the brain, and dropped the ratio of IL-17A and FoxP3 mRNA and protein in the brain or spinal cord at different stages. Conclusions: The study demonstrated that BSYSC had a strong neuroprotective effect on EAE mice. The protective mechanisms of BSYSC might be associated with mediating the regulation of Th17/Treg cells.
基金：
National Natural Science FoundationNational Natural Science Foundation of China [81072765, 81173237, 81273742, 81473640]; Scientific Research Key Program of Beijing Municipal Commission of EducationBeijing Municipal Commission of Education [KZ201310025023]; Program of Changcheng Scholars for the Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions [CITTCD20140329]
第一作者机构：[1]Capital Med Univ, Sch Tradit Chinese Med, Beijing 100069, Peoples R China;
通讯作者：
通讯机构：[2]Capital Med Univ, Beijing Tian Tan Hosp, Beijing 100050, Peoples R China;
推荐引用方式(GB/T 7714)：
Zheng Qi,Yang Tao,Fang Ling,et al.Effects of Bu Shen Yi Sui Capsule on Th17/Treg cytokines in C57BL/6 mice with experimental autoimmune encephalomyelitis[J].BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE.2015,15(1):-.doi:10.1186/s12906-015-0572-0.
APA：
Zheng, Qi,Yang, Tao,Fang, Ling,Liu, Lei,Liu, Haolong...&Wang, Lei.(2015).Effects of Bu Shen Yi Sui Capsule on Th17/Treg cytokines in C57BL/6 mice with experimental autoimmune encephalomyelitis.BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,15,(1)
MLA：
Zheng, Qi,et al."Effects of Bu Shen Yi Sui Capsule on Th17/Treg cytokines in C57BL/6 mice with experimental autoimmune encephalomyelitis".BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE 15..1(2015):-
