机构：[1]Capital Med Univ, Beijing Key Lab Pediat Hematol Oncol, Beijing 100045, Peoples R China;[2]Capital Med Univ, Natl Key Discipline Pediat, Minist Educ, Beijing 100045, Peoples R China;[3]Capital Med Univ, Key Lab Major Dis Children, Minist Educ, Beijing 100045, Peoples R China;[4]Capital Med Univ, Hematol Oncol Ctr, Beijing Childrens Hosp, Beijing 100045, Peoples R China;医技科室血液中心首都医科大学附属北京儿童医院[5]Univ Calif San Francisco, Sch Med, San Francisco, CA USA;[6]Capital Med Univ, Dept Immunol, Beijing 100069, Peoples R China;[7]Capital Med Univ, Beijing Key Lab Metab Disturbance Related Cardiov, Beijing 100069, Peoples R China;[8]Capital Med Univ, Dept Immunol, 10 Xi Tou Tiao, Beijing 100069, Peoples R China
Little is known about DNMT3A mutations in childhood acute lymphoblastic leukemia (ALL). We screened for DNMT3A mutations in exon 23 and its adjacent intron regions in diagnostic samples of 201 children with ALL. The cDNA samples from 82 patients were also sequenced to identify other mutations in the entire coding region. DNMT3A mutations were detected in exon 23 and its adjacent intron regions only in five patients (2.5%). There was only one mutation in exon 23 in two patients, respectively. In the other three patients, five intronic mutations were found. None of the mutations was found in the five corresponding complete remission samples. DNMT3A mutations were correlated with higher minimal residual disease at the end of remission induction (p = 0.078). Treatment outcome was obviously worse in patients with DNMT3A mutations than in other patients (p < 0.05). Thus, DNMT3A mutations can be found in a few children with ALL, and may have an adverse impact on prognosis.
基金：
Beijing Municipal Commission of EducationBeijing Municipal Commission of Education [KZ201410025021]; Beijing Municipal Administration of Hospitals Clinical medicine Development [ZY201404]; Beijing Health Qualified Personnel Program [2011-3-049]; NationalScience&TechnologyMajor Project of the 12th 5-Year Plan [2011ZX09302-007-01]; National Key Technologies Research & Development Program of the 11th 5-Year Plan [2007BAI04B03]; Beijing Municipal Science & Technology ProjectBeijing Municipal Science & Technology Commission [D0905001040431]; Beijing Novel ProgramBeijing Municipal Science & Technology Commission [2005B06]
第一作者机构：[1]Capital Med Univ, Beijing Key Lab Pediat Hematol Oncol, Beijing 100045, Peoples R China;[2]Capital Med Univ, Natl Key Discipline Pediat, Minist Educ, Beijing 100045, Peoples R China;[3]Capital Med Univ, Key Lab Major Dis Children, Minist Educ, Beijing 100045, Peoples R China;[4]Capital Med Univ, Hematol Oncol Ctr, Beijing Childrens Hosp, Beijing 100045, Peoples R China;
通讯作者：
通讯机构：[6]Capital Med Univ, Dept Immunol, Beijing 100069, Peoples R China;[7]Capital Med Univ, Beijing Key Lab Metab Disturbance Related Cardiov, Beijing 100069, Peoples R China;[8]Capital Med Univ, Dept Immunol, 10 Xi Tou Tiao, Beijing 100069, Peoples R China
推荐引用方式(GB/T 7714)：
Li Weijing,Gao Chao,Cui Lei,et al.DNMT3A mutations and prognostic significance in childhood acute lymphoblastic leukemia[J].LEUKEMIA & LYMPHOMA.2015,56(4):1066-1071.doi:10.3109/10428194.2014.947607.
APA：
Li, Weijing,Gao, Chao,Cui, Lei,Liu, Shuguang,Zhao, Xiaoxi...&Zhang, Quangeng.(2015).DNMT3A mutations and prognostic significance in childhood acute lymphoblastic leukemia.LEUKEMIA & LYMPHOMA,56,(4)
MLA：
Li, Weijing,et al."DNMT3A mutations and prognostic significance in childhood acute lymphoblastic leukemia".LEUKEMIA & LYMPHOMA 56..4(2015):1066-1071
