Aim: To investigate the status of DNA methylation in the Foxp3 promoter in pediatric ITP patients and assess the role of DNA methylation of Treg cells in the pathogenesis of ITP. Methods: Quantitative DNA methylation levels of Foxp3 promoter in pediatric ITP patients were detected by MassARRAY EpiTYPER. Methylation levels of Foxp3 promoter were analyzed in ITP patients and normal controls. Results: Significantly higher expression of CpG-2. CpG-3 and CpG-11.12 was observed in ITP patients compared to the controls. A subgroup analysis revealed that persistent and chronic ITP patients exhibited significantly higher CpG-6 expression than in the subgroup of newly diagnosed ITP patients. All patients who represented newly diagnosed ITP at admission were reclassified at later follow-up. In this follow-up subgroup analysis, there were significantly higher levels of CpG-6 in the persistent ITP group than that in the newly diagnosed ITP group. Conclusions: Our results indicate that defective Treg cell activity identified in ITP might be partially mediated through hypermethylation of CpG sites in the promoter region of Foxp3. (C) 2014 Published by Elsevier Inc. on behalf of American Society for Histocompatibility and Immunogenetics.
基金：
The authors would like to thank Dr. Daniel Edward Porter (Department of Orthopaedic Surgery, University of Edinburgh, Royal Hospital for Sick Children, UK) for critical review of the manuscript. This work was supported in part by grants from the National Natural Science Foundation of China – China (No. 81200351 , No. 81000228 ), Beijing Natural Science Foundation of China – China (No. 7112050 , No. 7122065 ). Beijing Municipal Administration of Hospitals Clinical medicine Development of special funding support – China, code ZY201404.
第一作者机构：[1]Capital Med Univ, Beijing Childrens Hosp, Hematol Oncol Ctr, Beijing Key Lab Pediat Hematol Oncol, Beijing 100045, Peoples R China;[2]Capital Med Univ, Beijing Childrens Hosp, Hematol Oncol Ctr, Natl Key Discipline Pediat,Minist Educ, Beijing 100045, Peoples R China;[3]Capital Med Univ, Beijing Childrens Hosp, Hematol Oncol Ctr, Key Lab Major Dis Children,Minist Educ, Beijing 100045, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Childrens Hosp, Hematol Oncol Ctr, Beijing Key Lab Pediat Hematol Oncol, Beijing 100045, Peoples R China;[2]Capital Med Univ, Beijing Childrens Hosp, Hematol Oncol Ctr, Natl Key Discipline Pediat,Minist Educ, Beijing 100045, Peoples R China;[3]Capital Med Univ, Beijing Childrens Hosp, Hematol Oncol Ctr, Key Lab Major Dis Children,Minist Educ, Beijing 100045, Peoples R China;[5]Capital Med Univ, Beijing Childrens Hosp, 56 Nanlishi Rd, Beijing 100045, Peoples R China
推荐引用方式(GB/T 7714)：
Chen Zhenping,Guo Zhenxing,Ma Jie,et al.Foxp3 methylation status in children with primary immune thrombocytopenia[J].HUMAN IMMUNOLOGY.2014,75(11):1115-1119.doi:10.1016/j.humimm.2014.09.018.
APA：
Chen, Zhenping,Guo, Zhenxing,Ma, Jie,Ma, Jingyao,Liu, Fuhong&Wu, Runhui.(2014).Foxp3 methylation status in children with primary immune thrombocytopenia.HUMAN IMMUNOLOGY,75,(11)
MLA：
Chen, Zhenping,et al."Foxp3 methylation status in children with primary immune thrombocytopenia".HUMAN IMMUNOLOGY 75..11(2014):1115-1119
