The functional connectivity of brain is a key point of brain network analysis. The BOLD (blood oxygen level dependent) fMRI (functional magnetic resonance imaging) signal is an effective projection signal of brain function. A dynamic method in resting-state (RS) functional connectivity analysis of brains is proposed in this paper. In contrast to traditional static method, a sliding window is used to separate whole period RS-BOLD signal into variable segments in time domain to rebuild a dynamic set of RS-BOLD and enlarge the sample size. It will enable the utilization of neural network classifier or other machine learning algorithms to analyze features and patterns. By training module from features extracted from brain network of glioma patients and normal people, it states 100% accuracy in glioma diagnosis. Besides, this dynamic analysis method also extracts 124 feature connections of glioma brain network with 70% confidence coefficient. By comparison, we also exploit brain network using general graph-based static method. It fails to reveal significant alternations between glioma and normal.