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Immunolocalization of NR1, NR2A, and PSD-95 in rat hippocampal subregions during postnatal development

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机构: [1]Capital Med Univ, Dept Anat, Being 100069, Peoples R China; [2]Capital Med Univ, Beijing Neurosurg Inst, Beijing 100050, Peoples R China; [3]Capital Med Univ, Beijing Tiantan Hosp, Beijing 100050, Peoples R China
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关键词: NR1 NR2A PSD-95 Hippocampus Development Rat

摘要:
Although the expression of NMDARs and synaptic-associated proteins has been widely studied, the temporospatial distribution of NMDAR subunits and synaptic proteins in different hippocampal subregions during postnatal development still lacks detailed information, and the relationship between NR1 or NR2 subunits and PSD-95 family proteins is controversial. In this study, we used immunofluorescent staining to assess NR1 or NR2A and PSD-95 expressions and the relationship between them in CA1, CA3, and DG of rat hippocampus on postnatal (P) days: P0, P4, P7, P10, P14, P21, P28, P56. The results showed that from PO to P56, NR1, NR2A, and PSD-95 expressions increased gradually, and the time points of their expression peak differed in CA1, CA3, and DG during postnatal development. Interestingly, although the expression of PSD-95 was positively correlated to both NR1 and NR2A, the NR1 and PSD-95 coexpressed puncta were greatest in CA3, while NR2A and PSD-95 coexpressed puncta were greatest in CA1, compared to other subregions. Surprisingly, at P21, among different strata of CA1, the area of highest expression of NR2A was dramatically changed from stratum pyramidale to stratum polymorphum and stratum moleculare, and returned to stratum pyramidale gradually on the later observed days again, indicating that P21 may be one critical timepoint during postnatal development in CA1. The specific temporospatial distribution pattern of NR1, NR2A, and PSD-95 might be related to the different physiological functions during postnatal development. Discovering the alteration of the relationship between PSD-95 and NMDAR subunits expression may be helpful for understanding mechanisms and therapy of neurodegenerative diseases. (C) 2011 Elsevier GmbH. All rights reserved.

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出版当年[2011]版:
大类 | 4 区 生物
小类 | 4 区 细胞生物学
最新[2023]版:
大类 | 4 区 生物学
小类 | 4 区 细胞生物学
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出版当年[2010]版:
Q4 CELL BIOLOGY
最新[2023]版:
Q4 CELL BIOLOGY

影响因子: 最新[2023版] 最新五年平均 出版当年[2010版] 出版当年五年平均 出版前一年[2009版] 出版后一年[2011版]

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第一作者机构: [1]Capital Med Univ, Dept Anat, Being 100069, Peoples R China;
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通讯机构: [1]Capital Med Univ, Dept Anat, Being 100069, Peoples R China;
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