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Anticoagulants affect matrix metalloproteinase 9 levels in blood samples of stroke patients and healthy controls

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机构： [a]Department of Neurology, Hannover Medical School, Carl-Neuberg-Straße 1, 30623 Hannover, Germany [b]Institute for Biostatistics, Hannover Medical School, Carl-Neuberg-Straße 1, 30623 Hannover, Germany [c]Institute for Clinical Chemistry, Hannover Medical School, Carl-Neuberg-Straße 1, 30623 Hannover, Germany [d]Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China [e]Theodor Bilharz Research Institute, Giza, Egypt

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关键词： Anticoagulant Matrix metalloproteinase-9 Mononuclear leukocytes Pre-analytic procedure Stroke

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Objectives: Matrix metalloproteinase-9 (MMP-9) represents a promising marker for acute stroke management. In clinical studies MMP-9 has been quantified by ELISA using differing protocols. We aimed to establish a valid protocol by evaluation of preanalytics. Design and methods: Blood from stroke patients (n = 28) and healthy controls (n = 28) was drawn into tubes containing different anticoagulants (EDTA, citrate, lithium-heparin (heparin) and heparin with proteinase inhibitors) and processed after 0, 60 and 240. min. MMP-9 plasma protein and mRNA from mononuclear leukocytes were determined. Results: In regard to anticoagulants used, samples showed different MMP-9 protein baseline values and kinetics. Stable MMP-9 protein concentrations were only measured from EDTA samples. Particularly in samples with proteinase inhibitors protein and mRNA concentrations increased over time. Kinetics did not differ between patients and controls. Conclusions: Preanalytics plays a key role for determination of MMP-9. EDTA seems to be a valid anticoagulant for MMP-9 protein measurement. © 2012 The Canadian Society of Clinical Chemists.

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出版当年[2011]版：

大类 | 4 区医学

小类 | 3 区医学实验技术

最新[2023]版：

大类 | 3 区医学

小类 | 3 区医学实验技术

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