机构：[a]National Key Laboratory for Agro-biotechnology, China Agriculture University, Beijing 100094, China[b]Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China首都医科大学附属天坛医院
The gene targeting vector pZJ was constructed according to the strategy of positive-negative selection, the Neo gene as the positive selected mark, the GFP gene as the negative selected mark. The pZJ was transferred into pig fetal fibroblast cells using the optimized lipotransfection method. After 7 days' G418 selection, 30 neo-positive cell clones were obtained. Among them, 12 were non-green ones, and 7 of which grew well. The genomic DNA samples from the 7 cell clones were identified by PCR method. The gene targeting vector pZJ was transferred into pig fetal fibroblast cells. 3 cell clones were site-specific recombinant by PCR. The pZJ was transferred into bovine fibroblast cells using the same lipotransfection method. After selection, two of the cell clones were identified as the site-specific recombinant by PCR method. The results provide a basis for Xenotransplantation and indicates that α-1,3-GT gene knock-out between species is feasible.
语种：
中文
第一作者：
推荐引用方式(GB/T 7714)：
Zhou J,Lin A,Chen Y.Porcine, bovine α-l,3-galactosyltransferase gene knock-out and HLA-G1 gene knock-in in somatic cells[J].2007,17(3):
