机构：[1]Ctr Dis Control & Prevent, Resp Dis Branch, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA;[2]Capital Univ Med Sci, Beijing Childrens Hosp, New York, NY 10045 USA首都医科大学附属北京儿童医院
Eighty-five cases community-acquired pneumonia (CAP) in children 5 years or younger, confirmed by chest X-ray, and 185 age-matched control patients with diarrhea or dermatitis from the Outpatient Department at Beijing Children's Hospital were enrolled into this study. Nasopharyngeal swab specimens were obtained from all subjects, Real-time PCR-based fluorescence assays were performed for Chlamydia pneumoniae and Mycoplasma pneumoniae. A nested PCR was also run for C. pneumoniae for comparison of assays. C. pneumoniae was found in 3 (3.5%) of CAP cases and in 4 (2.1%) of controls (P = 0.51). M. pneumoniae was found in 6 (7.1%) of CAP cases and in none of the controls (P = 0.001). The agreement rate of the 2 applied PCR methods used for C. pneumoniae detection was 98.5%. Our study demonstrates that M. pneumoniae may play a significant role in CAP affecting children up to 5 years in China, whereas C. pneumoniae in nasopharyngeal specimens was not associated with CAP in this age group. Published by Elsevier Inc.
基金：
This research was supported in part by an appointment to the Emerging Infectious Diseases (EID) Fellowship Program administered by the Association of Public Health Laboratories (APHL) and funded by the CDC. The authors thank the following persons at Beijing Children's Hospital for assistance with specimen collection, processing, and laboratory diagnosis: Y. Li, W. Gao, Y.J. Dong, J. Li, G.R. Zhang, and Y.Y. Hu. The authors also thank the radiologists who systematically interpreted the radiographs: G.Q. Sun, J.J. Zeng, S.Y. Xu, Z.F. Jiang, and D.X. Yao for their support and assistance. The authors thank S. Dowell, R. Garman, S. Newland, W.L. Thacker, S.B. Schwartz, R.F. Benson, V. Stevens, E. Brown, G.L. Xia, and E. Zell at CDC for their help and advice during the study. They also thank K. McCaustland for supplying the primers and probes for 16S rRNA gene analysis and technical support.
第一作者机构：[1]Ctr Dis Control & Prevent, Resp Dis Branch, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA;[2]Capital Univ Med Sci, Beijing Childrens Hosp, New York, NY 10045 USA
通讯作者：
通讯机构：[1]Ctr Dis Control & Prevent, Resp Dis Branch, Div Bacterial & Mycot Dis, Atlanta, GA 30333 USA;[2]Capital Univ Med Sci, Beijing Childrens Hosp, New York, NY 10045 USA
推荐引用方式(GB/T 7714)：
Liu G,Talkington DF,Fields BS,et al.Chlamydia pneumoniae and Mycoplasma pneumoniae in young children from China with community-acquired pneumonia[J].DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE.2005,52(1):7-14.doi:10.1016/j.diagmicrobio.2005.01.005.
APA：
Liu, G,Talkington, DF,Fields, BS,Levine, OS,Yang, YH&Tondella, MLC.(2005).Chlamydia pneumoniae and Mycoplasma pneumoniae in young children from China with community-acquired pneumonia.DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE,52,(1)
MLA：
Liu, G,et al."Chlamydia pneumoniae and Mycoplasma pneumoniae in young children from China with community-acquired pneumonia".DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE 52..1(2005):7-14
