Phosphatidylinositol-3-kinase (PI3K) has been identified in the expression of central sensitization after noxious inflammatory stimuli. However, its contribution in neuropathic pain remains to be determined. Here we address the role of PI3K signaling in central sensitization in a model of neuropathic pain, and propose a novel potential drug target for neuropathic pain. Chronic constriction injury (CCI) rat model was used in the study as the model for neuropathic pain. Western blotting, whole-cell patch clamp, and von Frey assay were performed to study biochemical, electrical, and behavioral changes in CCI rats, respectively. A steroid metabolite of the fungi (wortmannin) was used to block PI3K signaling and its effects on CCI rats were tested. PI3K/Akt signaling increased in the spinal cord L4-L6 sections in the CCI rats. CCI also facilitated miniature excitatory postsynaptic potential of dorsal horn substantia gelatinosa neurons, increased phosphorylation of glutamate receptor subunit GluA1 and synapsin at the synapse, and induced mechanic allodynia. Wortmannin reversed biochemical, electrical, and behavioral changes in CCI rats. This study is the first to show PI3K/Akt signaling is required for spinal central sensitization in the CCI neuropathic pain model.
第一作者机构：[1]Department of Anesthesiology, Daqing Longnan Hospital, No. 35 Patriotic Road, Ranghulu District, Daqing 163000, Heilongjiang, China
共同第一作者：
通讯作者：
通讯机构：[2]Department of Anesthesiology, Beijing Anzhen Hospital, Capital Medical University, No. 2 Anzhen Road, Beijing 100029, China
推荐引用方式(GB/T 7714)：
Liu Wei,Lv Yanling,Ren Facheng.PI3K/Akt Pathway is Required for Spinal Central Sensitization in Neuropathic Pain[J].CELLULAR AND MOLECULAR NEUROBIOLOGY.2018,38(3):747-755.doi:10.1007/s10571-017-0541-x.
APA：
Liu, Wei,Lv, Yanling&Ren, Facheng.(2018).PI3K/Akt Pathway is Required for Spinal Central Sensitization in Neuropathic Pain.CELLULAR AND MOLECULAR NEUROBIOLOGY,38,(3)
MLA：
Liu, Wei,et al."PI3K/Akt Pathway is Required for Spinal Central Sensitization in Neuropathic Pain".CELLULAR AND MOLECULAR NEUROBIOLOGY 38..3(2018):747-755
