A new method for investigating biological cell-protein interactions was developed by using a laser scanning confocal imaging-surface plasmon resonance (LSCI-SPR) system. Mouse normal IgG was modified on the SPR chip. The suspension mouse lymphocyte cancer cells (L5178Y cells) labeled by Hoechst33342 freely flowed into the surface of the SPR sensor chip. By changing the concentration of the cells, the fluorescence images and the SPR signal were synchronously recorded in real time. The red fluorescence points in the imaging region increased with increase in the concentration of the mouse lymphocyte cancer cells and fit well with the change in the SPR signal. Different suspending cells were chosen to investigate cell-protein interactions through antigen-antibody reactions on the biological cell surfaces through binding detection. This method has potential application in cell biology and pharmacology. (C) 2013 Elsevier B.V. All rights reserved.
基金：
National Nature Science Foundation of ChinaNational Natural Science Foundation of China [61378044]; National High Technology Research and Development Program of ChinaNational High Technology Research and Development Program of China [2009AA03Z318]; Chinese Academy of SciencesChinese Academy of Sciences [YZ200740]
通讯机构：[2]Capital Med Univ, Beijing Anzhen Hosp, Dept Pediat, Beijing 100029, Peoples R China
推荐引用方式(GB/T 7714)：
Zhang Hongyan,Yang Liquan,Zhou Bingjiang,et al.Investigation of biological cell-protein interactions using SPR sensor through laser scanning confocal imaging-surface plasmon resonance system[J].SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY.2014,121:381-386.doi:10.1016/j.saa.2013.10.100.
APA：
Zhang, Hongyan,Yang, Liquan,Zhou, Bingjiang,Wang, Xueliang,Liu, Guiying...&Wang, Pengfei.(2014).Investigation of biological cell-protein interactions using SPR sensor through laser scanning confocal imaging-surface plasmon resonance system.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,121,
MLA：
Zhang, Hongyan,et al."Investigation of biological cell-protein interactions using SPR sensor through laser scanning confocal imaging-surface plasmon resonance system".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 121.(2014):381-386
