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Abundance of the nasopharyngeal microbiome effects pertussis diagnosis and explains the sensitivity difference between bacterial culture and real-time PCR

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收录情况： ◇ SCIE

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机构： [a]Department of Neonatology, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China [b]Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China [c]Department of Microbial Research, WeHealthGene, Shenzhen, China [d]Department of Hematology and Oncology, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China

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关键词： Bordetella pertussis Culture Diagnosis Quantitative real-time PCR Sensitivity

摘要：

Quantitative real time PCR (qPCR)is used for pertussis diagnosis. The positive rate of qPCR is generally much higher than that of bacterial culture, which may cause confusion. The current study utilized the 16S ribosomal RNA (16S rRNA) sequencing to assess the correlation between conventional culture and qPCR and to explore the value of 16S rRNA in diagnosing pertussis. Nasopharyngeal swabs, collected from 102 children meeting clinical diagnostic criteria for pertussis, were subjected to Bordetella pertussis culture and qPCR. Bioinformatic microbiota analysis was based on 16S rRNA V3-V4 gene sequencing. Among 102 samples, 14 (13.7%) were culture-positive for Bordetella pertussis, while 61 (59.8%) were qPCR positive. Genus Bordetella was identified in 68 (66.7%) samples via 16S rRNA sequencing. When the relative abundance of Bordetella genus exceeded 0.70%, both qPCR and culture results were positive. Samples with a relative abundance of less than 0.20% exhibited positive qPCR and negative culture results. Samples with a low Bordetella abundance are the key factors underlying poor correlation between culture and qPCR results in laboratory tests. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.

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出版当年[2018]版：

大类 | 3 区医学

小类 | 3 区传染病学 3 区微生物学

最新[2023]版：

大类 | 3 区医学

小类 | 3 区传染病学 3 区微生物学

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出版当年[2017]版：

Q2 MICROBIOLOGY Q3 INFECTIOUS DISEASES

最新[2023]版：

Q2 INFECTIOUS DISEASES Q2 MICROBIOLOGY

影响因子： 3.7 最新[2023版] 3.5 最新五年平均 2.537 出版当年[2017版] 2.543 出版当年五年平均 2.727 出版前一年[2016版] 2.591 出版后一年[2018版]

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Abundance of the nasopharyngeal microbiome effects pertussis diagnosis and explains the sensitivity difference between bacterial culture and real-time PCR

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