Expression of hypoxia-inducible factor 1 alpha, glucose transporter 1, and hexokinase 2 in primary central nervous system lymphoma and the correlation with the biological behaviors
机构：[1]Department of Hematology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China[2]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China神经科系统神经外科首都医科大学宣武医院[3]Department of Orthopedics, Affiliated Hospital of Hebei University of Engineering, Handan, China[4]Neuroimaging Center, Beijing Tiantan Hospital, Capital Medical University, Beijing, China[5]Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
Background It has been indicated that abnormal glucose metabolism mediated by hypoxia-inducible factor 1 alpha (HIF-1 alpha) played an essential role in the development of solid tumor. However, there were rare studies about the role of them in primary central nervous system lymphoma (PCNSL). Objective To investigate the protein levels of HIF-1 alpha, glucose transporter 1 (GLUT1), and hexokinase 2 (HK2) in PCNSL and whether their levels are associated with prognostic factors. Methods Expression of HIF-1 alpha, GLUT1, and HK2 in 39 tumor tissues was evaluated by immunohistochemical stainning. The correlation of the expression of HIF-1 alpha with the protein level of GLUT1 and HK2 was investigated. In addition, the association between these protein expression levels and clinical parameters and prognosis was analyzed. Results In the tumor specimens of PCNSL, positive stainings of HIF-1 alpha, GLUT1, and HK2 were in 23 patients (58.97%), 25 patients (64.1%), and 26 patients (66.67%), respectively, which were associated with the expression level of lactic dehydrogenase (LDH), but not with age, gender, number of lesion, ECOG score, or deep structure. The expression of HIF-1 alpha was positively correlated with the expression of GLUT1 (p < .01,r = .749) and HK2 (p < .01,r = .787). Univariate analysis showed that upregulated GLUT1 was unfavorable predictors of progression-free survival (PFS) in PCNSL. The results of Cox proportional hazards model showed GLUT1 was significantly associated with shorter PFS (hazard ration: 5.65; 95% confidence interval: 1.23-25.84;p = .026). Conclusions This study indicated that there was a hypoxic microenvironment and HIF-1 alpha was involved in the regulation of glycolysis pathway in PCNSL. GLUT1 might be a potential marker for shorter PFS in PCNSL.
基金：
Beijing Natural Science Foundation of ChinaBeijing Natural Science Foundation [7172071]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81272842]
第一作者机构：[1]Department of Hematology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
通讯作者：
通讯机构：[*1]Department of Hematology, Beijing Tiantan Hospital, Capital Medical University, No. 119 South Sihuan West Road, Fengtai District, Beijing 100070, China.
推荐引用方式(GB/T 7714)：
Shen Na,Wang Yaming,Sun Xuefei,et al.Expression of hypoxia-inducible factor 1 alpha, glucose transporter 1, and hexokinase 2 in primary central nervous system lymphoma and the correlation with the biological behaviors[J].BRAIN AND BEHAVIOR.2020,10(8):doi:10.1002/brb3.1718.
APA：
Shen, Na,Wang, Yaming,Sun, Xuefei,Bai, Xueyan,He, Jinglan...&Liu, Yuanbo.(2020).Expression of hypoxia-inducible factor 1 alpha, glucose transporter 1, and hexokinase 2 in primary central nervous system lymphoma and the correlation with the biological behaviors.BRAIN AND BEHAVIOR,10,(8)
MLA：
Shen, Na,et al."Expression of hypoxia-inducible factor 1 alpha, glucose transporter 1, and hexokinase 2 in primary central nervous system lymphoma and the correlation with the biological behaviors".BRAIN AND BEHAVIOR 10..8(2020)
