机构：[1]Department of Cardiology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China内科系统心脏科（内科专业）首都医科大学宣武医院[2]Department of Physiology and Pathophysiology, Capital Medical University, Beijing, China[3]Department of Pathology, Beijing Youan Hospital, Capital Medical University, Beijing, China[4]Beijing Anzhen Hospital, Capital Medical University, Beijing, China首都医科大学附属安贞医院[5]Department of Animal Experimental Center, Fuwai Hospital, National Center for Cardiovascular Disease, China Academy of Medical Sciences, Beijing, China
The objective of this study was to investigate the effects of glycaemic variability (GV) on intimal hyperplasia and plaque stability after coronary stenting via autophagy-mediated G3BP1/NLRP3 inflammasome signalling.
In the clinical study, between July 2017 and December 2017, 95 patients with acute myocardial infarction (AMI) and diabetes mellitus (DM) comorbidity received stent implantation. The patients were followed up for 2 years after discharge. The patients were divided into a low-GV (n=61) and high-GV (n=34) group, and the incidence of recurrent AMI was measured. In the animal study, thirteen pigs were divided into a sham (n=3), low-GV DM (n=5) and high-GV DM group (n=5). Intima samples were analysed by optical coherence tomography 22 weeks after coronary stenting. Becn1, LC3B, p62, G3BP1 and NLRP3 protein levels in the intima were examined by western blot. In vitro experiments with THP-1 cells were also conducted.
In the high-GV group, patients exhibited a higher recurrent AMI, greater neointimal thickness, increased p62 and NLRP3 expression, and decreased Becn1, LC3B and G3BP1 expression compared with the low-GV group (P<0.05). The effects of high GV could be abolished by rapamycin but were aggravated by 3-methyladenine.
GV might impact the intimal hyperplasia and plaque stability via autophagy-mediated G3BP1/NLRP3 inflammasome signalling. GV and the autophagy-mediated G3BP1/NLRP3 inflammasome may be promising targets for the treatment of coronary heart disease.
2020 Annals of Translational Medicine. All rights reserved.
基金：
the National Natural Science Foundation of China (Grant number 81770344), the National Clinical Research Center for Geriatric Diseases, the Beijing Key Clinical Speciality Development Project and the China Young and Middle-aged Clinical Research VG fund (Grant number 2017-CCA-VG-043).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|2 区医学
小类|2 区医学：研究与实验2 区肿瘤学
最新[2023]版：
无
第一作者：
第一作者机构：[1]Department of Cardiology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China
通讯作者：
通讯机构：[*1]Department of Physiology and Pathophysiology, Capital Medical University, Beijing China.[*2]Department of Cardiology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China
推荐引用方式(GB/T 7714)：
Xia Jinggang,Zhang Jia,Chang Jing,et al.The effects of glycaemic variability on intimal hyperplasia and plaque stability after stenting via autophagy-mediated G3BP1/NLRP3 inflammasome.[J].Annals of translational medicine.2020,8(21):1388.doi:10.21037/atm-20-4818.
APA：
Xia Jinggang,Zhang Jia,Chang Jing,Tian Yi,Li Jubo...&Yin Chunlin.(2020).The effects of glycaemic variability on intimal hyperplasia and plaque stability after stenting via autophagy-mediated G3BP1/NLRP3 inflammasome..Annals of translational medicine,8,(21)
MLA：
Xia Jinggang,et al."The effects of glycaemic variability on intimal hyperplasia and plaque stability after stenting via autophagy-mediated G3BP1/NLRP3 inflammasome.".Annals of translational medicine 8..21(2020):1388
